Search: onr:"swepub:oai:DiVA.org:liu-98852" >
AP-1 DNA-binding ac...
AP-1 DNA-binding activity is inhibited by selenite and selenodiglutathione
-
- Spyrou, Giannis (author)
- Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
-
- Björnstedt, M. (author)
- Karolinska Institutet
-
- Kumar, S. (author)
- Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
-
show more...
-
- Holmgren, A. (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Elsevier, 1995
- 1995
- English.
-
In: FEBS Letters. - : Elsevier. - 0014-5793 .- 1873-3468. ; 368:1, s. 59-63
- Related links:
-
http://www.sciencedi...
-
show more...
-
https://urn.kb.se/re...
-
http://kipublication...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- The binding of the transcription factor AP-1 to DNA has been shown to be modulated by redox control mechanisms. Selenite and selenodiglutathione (GS-Se-SG), inhibit mammalian cell growth and are efficient oxidants of reduced thioredoxin and reduced thioredoxin reductase. Here, we report that selenite and GS-Se-SG efficiently inhibited AP-1 DNA-binding in nuclear extracts from 3B6 lymphocytes. A GS-Se-SG concentration of 0.75 microM resulted in 50% inhibition of AP-1 DNA-binding, whereas the same effect was achieved with 7.5 microM selenite. Nuclear extracts prepared from human 3B6 lymphocytes exposed for 4 h to 10 microM selenite showed a 50% reduction of AP-1 binding. These data suggest that selenite and selenodiglutathione inactivate the AP-1 factor and provide a mechanism by which selenium compounds inhibit cell growth.
Keyword
- Selenium; Selenodiglutathione; Redox regulation; AP-1
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database