Search: onr:"swepub:oai:DiVA.org:liu-99406" >
Faecalibacterium pr...
Faecalibacterium prausnitzii supernatant improves intestinal barrier function in mice DSS colitis
-
- Carlsson, Anders H. (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Yakymenko, Olena (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Olivier, Isabelle (author)
- External - unknown
-
show more...
-
- Håkansson, Fathima (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Postma, Emily (author)
- External - unknown
-
- Keita, Asa V. (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Soderholm, Johan D. (author)
- Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet,Kirurgiska kliniken US
-
show less...
-
(creator_code:org_t)
- 2013-08-26
- 2013
- English.
-
In: Scandinavian Journal of Gastroenterology. - : Informa Healthcare. - 0036-5521 .- 1502-7708. ; 48:10, s. 1136-1144
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- Objective. The intestinal microbiota plays a substantial role in the pathogenesis of inflammatory bowel disease (IBD). Faecalibacterium prausnitzii (FP) is underrepresented in IBD patients and have been suggested to have anti-inflammatory effects in mice. Increased intestinal permeability is common in IBD but the relationship between FP and intestinal barrier function has not been investigated. Our aim was to study treatment with FP supernatant on intestinal barrier function in a dextran sodium sulfate (DSS) colitis mice model. Material and methods. C57BL/6 mice received 3% DSS in tap water ad libitum during five days to induce colitis. From day 3 the mice received a daily gavage with FP supernatant or broth during seven days. Ileum and colon were mounted in Ussing chambers for permeability studies with Cr-51-EDTA and Escherichia coli K-12. Colon was saved for Western blot analyses of tight junction proteins. Results. DSS-treated mice showed significant weight loss and colon shortening. Gavage with FP supernatant resulted in a quicker recovery after DSS treatment and less extensive colonic shortening. Ileal mucosa of DSS mice showed a significant increase in Cr-51-EDTA-passage compared to controls. Cr-51-EDTA passage was significantly decreased in mice receiving FP supernatant. No significant differences were observed in passage of E. coli K12. Western blots showed a trend to increased claudin-1 and claudin-2 expressions in DSS mice. Conclusions. Supernatant of FP enhances the intestinal barrier function by affecting paracellular permeability, and may thereby attenuate the severity of DSS-induced colitis in mice. These findings suggest a potential role of FP in the treatment of IBD.
Keyword
- dextran sodium sulfate
- inflammatory bowel disease
- permeability
- probiotics
- tight junctions
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database