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Activity in MCF-7 E...
Activity in MCF-7 Estrogen-sensitive Breast Cancer Cells of Capsicodendrin from Cinnamosma fragrans
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- Acuña, Ulyana Muñoz (author)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Ohio State Univ, USA
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- Ezzone, Nathan (author)
- Ohio State Univ, USA
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- Rakotondraibe, L. Harinantenaina (author)
- Ohio State Univ, USA
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- De Blanco, Esperanza J. Carcache (author)
- Ohio State Univ, USA
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(creator_code:org_t)
- 2021-11-30
- 2021
- English.
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In: Anticancer Research. - : International Institute of Anticancer Research. - 0250-7005 .- 1791-7530. ; 41:12, s. 5935-5944
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https://doi.org/10.2...
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Abstract
Subject headings
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- Background/Aim: Effect of capsicodendrin on the NF-KB pathway was studied in MCF-7 cancer cells. Materials and Methods: The transcription factor assay was used to screen for NF-KB activity. The effect on IKK beta, ICAM-1, and caspase-7 were studied using western blot. Caspase-1 was studied using Promega Caspase-Glo (R) assay. Reactive oxygen species (ROS) were detected using the fluorescent probe DCFH-DA. The potentiometric dye JC-1 was used to assess mitochondrial membrane potential (Delta psi m) and the cell cycle was examined using a fluorescence-activated cell sorter. Results: NF-kappa B p65 inhibitory effect was IC50=8.6 mu M and cytotoxic activity was IC50=7.5 mu M. The upstream IKK and the downstream ICAM-1 were down-regulated. Sub G1-phase population increased to 81% after 12 h of treatment with capsicodendrin (10 mu M) and there was no loss of Delta psi M. Conclusion: Increased levels of intracellular ROS promoted activity of caspase-1 and induced cell death in MCF-7 cells. Capsicodendrin may be a future anticancer agent that prevents the progression of metastatic breast cancer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- Capsicodendrin
- MCF-7
- NF-kappa B
- IKK beta
- ICAM-1
- ROS
- caspase-7
- caspase-1
- Pharmacology
- Farmakologi
- Biokemi
- Biochemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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