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  • Banas, IndraUniversity of Duisburg-Essen, Germany (author)

Spatio-functional organization in virocells of small uncultivated archaea from the deep biosphere

  • Article/chapterEnglish2023

Publisher, publication year, extent ...

  • Springer Nature,2023
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:lnu-123459
  • https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-123459URI
  • https://doi.org/10.1038/s41396-023-01474-1DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Despite important ecological roles posited for virocells (i.e., cells infected with viruses), studying individual cells in situ is technically challenging. We introduce here a novel correlative microscopic approach to study the ecophysiology of virocells. By conducting concerted virusFISH, 16S rRNA FISH, and scanning electron microscopy interrogations of uncultivated archaea, we linked morphologies of various altiarchaeal cells to corresponding phylogenetic signals and indigenous virus infections. While uninfected cells exhibited moderate separation between fluorescence signals of ribosomes and DNA, virocells displayed complete cellular segregation of chromosomal DNA from viral DNA, the latter co-localizing with host ribosome signals. A similar spatial separation was observed in dividing cells, with viral signals congregating near ribosomes at the septum. These observations suggest that replication of these uncultivated viruses occurs alongside host ribosomes, which are used to generate the required proteins for virion assembly. Heavily infected cells sometimes displayed virus-like particles attached to their surface, which agree with virus structures in cells observed via transmission electron microscopy. Consequently, this approach is the first to link genomes of uncultivated viruses to their respective structures and host cells. Our findings shed new light on the complex ecophysiology of archaeal virocells in deep subsurface biofilms and provide a solid framework for future in situ studies of virocells.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Esser, Sarah P.University of Duisburg-Essen, Germany (author)
  • Turzynski, VictoriaUniversity of Duisburg-Essen, Germany (author)
  • Soares, AndréUniversity of Duisburg-Essen, Germany (author)
  • Novikova, PolinaUniversity of Luxembourg, Luxembourg (author)
  • May, PatrickUniversity of Luxembourg, Luxembourg (author)
  • Moraru, CristinaUniversity of Luxembourg, Luxembourg;Carl-von-Ossietzky-University Oldenburg, Germany (author)
  • Hasenberg, MikeImaging Center Essen, Germany (author)
  • Rahlff, JaninaLinnéuniversitetet,Institutionen för biologi och miljö (BOM),University Duisburg-Essen, Germany,Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)jaraad (author)
  • Wilmes, PaulUniversity of Luxembourg, Luxembourg (author)
  • Klingl, AndreasBiocenter LMU Munich, Germany (author)
  • Probst, Alexander J.University of Duisburg-Essen, Germany (author)
  • University of Duisburg-Essen, GermanyUniversity of Luxembourg, Luxembourg (creator_code:org_t)

Related titles

  • In:The ISME Journal: Springer Nature17, s. 1789-17921751-73621751-7370

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