Long-Term Storage of Surface-Adsorbed Protein Machines

Albet-Torres, NuriaLinnéuniversitetet,Institutionen för naturvetenskap, NV (författare)

The effective and simple long-term storage of complex functional proteins is critical in achieving commercially viable biosensors. This issue is particularly challenging in recently proposed types of nanobiosensors, where molecular-motor-driven transportation substitutes microfluidics and forms the basis for novel detection schemes. Importantly, therefore, we here describe that delicate heavy meromyosin (HMM)-based nanodevices (HMM motor fragments adsorbed to silanized surfaces and actin bound to HMM) fully maintain their function when stored at -20 degrees C for more than a month. The mechanisms for the excellent preservation of acto-HMM motor function upon repeated freeze thaw cycles are discussed. The results are important to the future commercial implementation of motor-based nanodevices and are of more general value to the long-term storage of any protein-based bionanodevice.

Månsson, AlfLinnéuniversitetet,Institutionen för naturvetenskap, NV(Swepub:lnu)nmaal (författare)
LinnéuniversitetetInstitutionen för naturvetenskap, NV (creator_code:org_t)

Ingår i:Langmuir: American Chemical Society (ACS)27:11, s. 7108-71120743-74631520-5827

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