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Silk matrices promote formation of insulin-secreting islet-like clusters
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- Dekki Shalaly, Nancy (author)
- KTH,Proteinteknologi,KTH Royal Institute of Technology
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- Ria, Massimiliano (author)
- Karolinska Institutet
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- Johansson, Ulrika, 1974- (author)
- KTH,Proteinteknologi,Royal Institute of Technology (KTH),KTH Royal Institute of Technology
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- (creator_code:org_t)
- Elsevier BV, 2016
- 2016
- English.
- In: Biomaterials. - : Elsevier BV. - 0142-9612 .- 1878-5905. ; 90, s. 50-61
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Ex vivo expansion of endocrine cells constitutes an interesting alternative to be able to match the unmet need of transplantable pancreatic islets. However, endocrine cells become fragile once removed from their extracellular matrix (ECM) and typically become senescent and loose insulin expression during conventional 2D culture. Herein we develop a protocol where 3D silk matrices functionalized with ECM-derived motifs are used for generation of insulin-secreting islet-like clusters from mouse and human primary cells. The obtained clusters were shown to attain an islet-like spheroid shape and to maintain functional insulin release upon glucose stimulation in vitro. Furthermore, in vivo imaging of transplanted murine clusters showed engraftment with increasing vessel formation during time. There was no sign of cell death and the clusters maintained or increased in size throughout the period, thus suggesting a suitable cluster size for transplantation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- Recombinant spider silk
- Pancreatic islets
- Islet-like clusters
- Insulin release
- In vivo imaging
- Vascularization
- Natural Science
- Naturvetenskap
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- ref (subject category)
- art (subject category)
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