Silk matrices promote formation of insulin-secreting islet-like clusters

Dekki Shalaly, NancyKTH,Proteinteknologi,KTH Royal Institute of Technology (author)

QC 20160520
Ex vivo expansion of endocrine cells constitutes an interesting alternative to be able to match the unmet need of transplantable pancreatic islets. However, endocrine cells become fragile once removed from their extracellular matrix (ECM) and typically become senescent and loose insulin expression during conventional 2D culture. Herein we develop a protocol where 3D silk matrices functionalized with ECM-derived motifs are used for generation of insulin-secreting islet-like clusters from mouse and human primary cells. The obtained clusters were shown to attain an islet-like spheroid shape and to maintain functional insulin release upon glucose stimulation in vitro. Furthermore, in vivo imaging of transplanted murine clusters showed engraftment with increasing vessel formation during time. There was no sign of cell death and the clusters maintained or increased in size throughout the period, thus suggesting a suitable cluster size for transplantation.

Ria, MassimilianoKarolinska Institutet (author)
Johansson, Ulrika,1974-KTH,Proteinteknologi,Royal Institute of Technology (KTH),KTH Royal Institute of Technology(Swepub:slu)48573 (author)
Åvall, KarinKarolinska Institutet (author)
Berggren, Per-OlofKarolinska Institutet (author)
Hedhammar, MySwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,KTH,Proteinteknologi,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Royal Institute of Technology (KTH),KTH Royal Institute of Technology ; Swedish University of Agricultural Sciences(Swepub:slu)46815 (author)
KTHProteinteknologi (creator_code:org_t)
Sveriges lantbruksuniversitet

By the author/editor: Dekki Shalaly, N ...; Ria, Massimilian ...; Johansson, Ulrik ...; Åvall, Karin; Berggren, Per-Ol ...; Hedhammar, My

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

By the university: Linnaeus University; Royal Institute of Technology; Karolinska Institutet; Swedish University of Agricultural Sciences

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