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Novel ATP-cone-driv...
Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit
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- Rozman Grinberg, Inna (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University
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- Lundin, Daniel, 1965- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University
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- Hasan, Mahmudul (author)
- Stockholm University,Lunds universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Lund University, Sweden,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.;Lund Univ, Dept Biochem & Struct Biol, Lund, Sweden.,Stockholm University ; Lund University,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Crona, Mikael (author)
- Swedish Ophan Biovitrum AB, Stockholm, Sweden.
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- Rao Jonna, Venkateswara (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.,Umeå University
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- Loderer, Chrishtoph (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University
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- Sahlin, Margareta (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University
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- Markova, Natalia (author)
- Malvern Instruments Inc, Malvern, Sweden.
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- Borovok, Ilya (author)
- Tel Aviv University, Israel,Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, Tel Aviv, Israel.
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- Berggren, Gustav (author)
- Uppsala universitet,Molekylär biomimetik,Uppsala University
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- Hofer, Anders (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.,Umeå University
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- Logan, Derek T. (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Sjöberg, Britt-Marie (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University
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(creator_code:org_t)
- ELIFE SCIENCES PUBLICATIONS LTD, 2018
- 2018
- English.
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In: eLIFE. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 7
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Abstract
Subject headings
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- Ribonucleotide reductases (RNRs) are key enzymes in DNA metabolism, with allosteric mechanisms controlling substrate specificity and overall activity. In RNRs, the activity master-switch, the ATP-cone, has been found exclusively in the catalytic subunit. In two class I RNR subclasses whose catalytic subunit lacks the ATP-cone, we discovered ATP-cones in the radical-generating subunit. The ATP-cone in the Leeuwenhoekiella blandensis radical-generating subunit regulates activity via quaternary structure induced by binding of nucleotides. ATP induces enzymatically competent dimers, whereas dATP induces non-productive tetramers, resulting in different holoenzymes. The tetramer forms by interactions between ATP-cones, shown by a 2.45 A crystal structure. We also present evidence for an (MnMnIV)-Mn-III metal center. In summary, lack of an ATP-cone domain in the catalytic subunit was compensated by transfer of the domain to the radical-generating subunit. To our knowledge, this represents the first observation of transfer of an allosteric domain between components of the same enzyme complex.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Biokemi
- Biochemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Rozman Grinberg, ...
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Lundin, Daniel, ...
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Hasan, Mahmudul
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Crona, Mikael
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Rao Jonna, Venka ...
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Loderer, Chrisht ...
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show more...
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Sahlin, Margaret ...
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Markova, Natalia
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Borovok, Ilya
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Berggren, Gustav
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Hofer, Anders
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Logan, Derek T.
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Sjöberg, Britt-M ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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eLIFE
- By the university
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Linnaeus University
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Umeå University
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Stockholm University
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Uppsala University
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Lund University