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Regeneration of Ass...
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Rahman, Mohammad A.Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Lund University, Sweden
(author)
Regeneration of Assembled, Molecular-Motor-Based Bionanodevices
- Article/chapterEnglish2019
Publisher, publication year, extent ...
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2019-09-05
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American Chemical Society (ACS),2019
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:lnu-89868
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https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-89868URI
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https://doi.org/10.1021/acs.nanolett.9b02738DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-311325URI
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https://lup.lub.lu.se/record/86dd7ed6-6c46-4d17-a693-0b5e75629ddcURI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20220426
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The guided gliding of cytoskeletal filaments, driven by biomolecular motors on nano/microstructured chips, enables novel applications in biosensing and biocomputation. However, expensive and time-consuming chip production hampers the developments. It is therefore important to establish protocols to regenerate the chips, preferably without the need to dismantle the assembled microfluidic devices which contain the structured chips. We here describe a novel method toward this end. Specifically, we use the small, nonselective proteolytic enzyme, proteinase K to cleave all surface-adsorbed proteins, including myosin and kinesin motors. Subsequently, we apply a detergent (5% SDS or 0.05% Triton X100) to remove the protein remnants. After this procedure, fresh motor proteins and filaments can be added for new experiments. Both, silanized glass surfaces for actin-myosin motility and pure glass surfaces for microtubule-kinesin motility were repeatedly regenerated using this approach. Moreover, we demonstrate the applicability of the method for the regeneration of nano/microstructured silicon-based chips with selectively functionalized areas for supporting or suppressing gliding motility for both motor systems. The results substantiate the versatility and a promising broad use of the method for regenerating a wide range of protein-based nano/microdevices.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Reuther, CordulaTech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany,Dresden University of Technology,Max Planck Institute of Molecular Cell Biology and Genetics
(author)
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Lindberg, Frida W.Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ftf-fdl
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Mengoni, MartinaTech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany,Max Planck Institute of Molecular Cell Biology and Genetics,Dresden University of Technology
(author)
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Salhotra, AseemLinnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, 39182, Sweden; NanoLund, Lund University, Lund, 22100, Sweden(Swepub:kth)u1bx7doc
(author)
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Heldt, GeorgFraunhofer Inst Elect Nano Syst, Germany,Fraunhofer Institute for Electronic Nanosystems (ENAS)
(author)
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Linke, HeinerLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ftf-hle
(author)
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Diez, StefanTech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany,Dresden University of Technology,Max Planck Institute of Molecular Cell Biology and Genetics
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Månsson, AlfLinnaeus University,Lunds universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Avancerade material,Lund University, Sweden,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Other operations, LTH,Faculty of Engineering, LTH(Swepub:lu)al0631ma
(author)
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LinnéuniversitetetInstitutionen för kemi och biomedicin (KOB)
(creator_code:org_t)
Related titles
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In:Nano Letters: American Chemical Society (ACS)19:10, s. 7155-71631530-69841530-6992
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