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Selective and marke...
Selective and marked decrease of complement receptor C5aR2 in human thoracic aortic aneurysms : a dysregulation with potential inflammatory effects
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- Holt, Margrethe (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
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- Seim, Bjorn E. (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
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- Ogaard, Jonas (author)
- Oslo Univ Hosp, Norway
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- Olsen, Maria B. (author)
- Oslo Univ Hosp, Norway
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- Woldbaek, Per R. (author)
- Oslo Univ Hosp Ulleval, Norway
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- Kvitting, John-Peder Escobar (author)
- Oslo Univ Hosp, Norway
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- Aukrust, Pal (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
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- Yndestad, Arne (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
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- Mollnes, Tom Eirik (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway;Nordland Hosp, Norway;Univ Tromsø, Norway
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- Nilsson, Per H., 1980- (author)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Avancerade material,Univ Oslo, Norway,Linnaeus Ctr Biomat Chem, BMC;HoRB
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- Louwe, Mieke C. (author)
- Oslo Univ Hosp, Norway
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- Ranheim, Trine (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
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(creator_code:org_t)
- 2019-11-10
- 2019
- English.
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In: Open heart. - : BMJ Publishing Group Ltd. - 2053-3624. ; 6:2, s. 1-8
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https://doi.org/10.1...
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https://openheart.bm...
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Abstract
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- Objective The aetiology of thoracic aortic aneurysm (TAA) is largely unknown, but inflammation is likely to play a central role in the pathogenesis. In this present study, we aim to investigate the complement receptors in TAA. Methods Aortic tissue and blood from 31 patients with non-syndromic TAA undergoing thoracic aortic repair surgery were collected. Aortic tissue and blood from 36 patients with atherosclerosis undergoing coronary artery bypass surgery or aortic valve replacement were collected and served as control material. The expression of the complement anaphylatoxin receptors C3aR1, C5aR1 and C5aR2 in aortic tissue were examined by quantitative RT-PCR and C5aR2 protein by immunohistochemistry. Colocalisation of C5aR2 to different cell types was analysed by immunofluorescence. Complement activation products C3bc and sC5b-9 were measured in plasma. Results Compared with controls, TAA patients had substantial (73%) downregulated gene expression of C5aR2 as seen both at the mRNA (p=0.005) level and protein (p=0.03) level. In contrast, there were no differences in the expression of C3aR1 and C5aR1 between the two groups. Immunofluorescence examination showed that C5aR2 was colocalised to macrophages and T cells in the aortic media. There were no differences in the degree of systemic complement activation between the two groups. Conclusion Our findings suggest downregulation of the C5aR2, regarded to act mainly anti-inflammatory, in electively operated TAA as compared with non-aneurysmatic aortas of patients with aortic stenosis and/or coronary artery disease. This may tip the balance towards a relative increase in the inflammatory responses induced by C5aR1 and thus enhance the inflammatory processes in TAA.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Keyword
- Medicin
- Medicine
Publication and Content Type
- ref (subject category)
- art (subject category)
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Open heart
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- By the author/editor
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Holt, Margrethe
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Seim, Bjorn E.
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Ogaard, Jonas
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Olsen, Maria B.
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Woldbaek, Per R.
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Kvitting, John-P ...
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show more...
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Aukrust, Pal
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Yndestad, Arne
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Mollnes, Tom Eir ...
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Nilsson, Per H., ...
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Louwe, Mieke C.
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Ranheim, Trine
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cardiac and Card ...
- Articles in the publication
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Open heart
- By the university
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Linnaeus University