onr:"swepub:oai:DiVA.org:ltu-11309"
Search: onr:"swepub:oai:DiVA.org:ltu-11309" > Effects of snap-fre...
- 1 of 1
- Previous record
- Next record
- To hitlist
Effects of snap-freezing and near-infrared laser illumination on porcine prostate tissue as measured by Raman spectroscopy
- Candefjord, Stefan (author)
-
- Ramser, Kerstin (author)
- Luleå tekniska universitet,Signaler och system
- Lindahl, Olof (author)
- (creator_code:org_t)
- Royal Society of Chemistry (RSC), 2009
- 2009
- English.
- In: The Analyst. - : Royal Society of Chemistry (RSC). - 0003-2654 .- 1364-5528. ; 134:9, s. 1815-1821
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Most Raman spectroscopic studies on tissue are performed in vitro. To assure that the results are applicable to in vivo examinations, preparation protocols and measurement procedures of tissue for in vitro studies should preserve tissue characteristics close to the native state. This study had two aims. The first was to elucidate if photoinduced effects arise during 5 minutes' continuous illumination of tissue with an 830 nm laser at an irradiance of 3 × 1010 W/m2. The second was to investigate the effects of snap-freezing of porcine prostate tissue in liquid nitrogen and subsequent storage at -80 °C, by means of multivariate analysis. 830 nm laser illumination of the specified irradiance did not affect the Raman spectra. A decrease of the spectral background was observed, likely due to photobleaching of tissue fluorophores. Snap-freezing and subsequent storage at -80 °C gave rise to subtle but significant alterations in Raman spectra, most likely related to changes in the protein conformations
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Annan medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Other Medical Engineering (hsv//eng)
Keyword
- Medical Engineering for Healthcare
- Medicinsk teknik för hälsovård
- Centre - Centre for Biomedical Engineering and Physics (CMTF)
- Centrumbildning - Centrum för medicinsk teknik och fysik (CMTF)
Publication and Content Type
- ref (subject category)
- art (subject category)
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Candefjord, Stef ...
- Ramser, Kerstin
- Lindahl, Olof
- About the subject
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Medical Engineer ...
- and Other Medical En ...
- Articles in the publication
- The Analyst
- By the university
- Luleå University of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
