Optimization of β-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose

Christakopoulos, Paul (author)

(creator_code:org_t)

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Purified β-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15–20 % of the initial substrate concentration. These results suggested that β-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.

By the author/editor: Christakopoulos, ...; Kekos, Dimitris; Macris, Basil J.; Goodenough, Pete ...; Bhat, Mahalinges ...

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