Amyloid Hydrogen Bonding Polymorphism Evaluated by 15N{17O}REAPDOR Solid-State NMR and Ultra-High Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Wei, JuanDepartment of Chemistry and Department of Physics, University of Warwick, Coventry (author)

Validerad; 2016; Nivå 2; 20160414 (andbra)
A combined approach, using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and solid-state NMR (Nuclear Magnetic Resonance), shows a high degree of polymorphism exhibited by Aβ species in forming hydrogen-bonded networks. Two Alzheimer’s Aβ peptides, Ac-Aβ16–22-NH2 and Aβ11–25, selectively labeled with 17O and 15N at specific amino acid residues were investigated. The total amount of peptides labeled with 17O as measured by FTICR-MS enabled the interpretation of dephasing observed in 15N{17O}REAPDOR solid-state NMR experiments. Specifically, about one-third of the Aβ peptides were found to be involved in the formation of a specific >C═17O···H–15N hydrogen bond with their neighbor peptide molecules, and we hypothesize that the rest of the molecules undergo ± n off-registry shifts in their hydrogen bonding networks.

Antzutkin, OlegLuleå tekniska universitet,Kemiteknik(Swepub:ltu)olan (author)
Filippov, AndreiLuleå tekniska universitet,Kemiteknik(Swepub:ltu)andfil (author)
Iuga, DinuDepartment of Physics, Warwick University, Coventry, Department of Chemistry and Department of Physics, University of Warwick, Coventry (author)
Lam, Pui YiuDepartment of Chemistry and Department of Physics, University of Warwick, Coventry (author)
Barrow, Mark P.Department of Chemistry and Department of Physics, University of Warwick, Coventry (author)
Dupree, RayUniversity of Warwick, Department of Physics, Warwick University, Coventry, Department of Chemistry and Department of Physics, University of Warwick, Coventry (author)
Brown, Steven P.Department of Physics, Warwick University, Coventry, Department of Chemistry and Department of Physics, University of Warwick, Coventry (author)
O'Connor, Peter B.Department of Chemistry and Department of Physics, University of Warwick, Coventry (author)
Department of Chemistry and Department of Physics, University of Warwick, CoventryKemiteknik (creator_code:org_t)

In:Biochemistry: American Chemical Society (ACS)55:14, s. 2065-20680006-29601520-4995

By the author/editor: Wei, Juan; Antzutkin, Oleg; Filippov, Andrei; Iuga, Dinu; Lam, Pui Yiu; Barrow, Mark P.; show more...; Dupree, Ray; Brown, Steven P.; O'Connor, Peter ...; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Physical Chemist ...

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