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Optimized synthesis...
Optimized synthesis of novel prenyl ferulate performed by feruloyl esterases from Myceliophthora thermophila in microemulsions
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- Antonopoulou, Io (författare)
- Luleå tekniska universitet,Kemiteknik
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- Leonov, Laura (författare)
- DuPont Industrial Biosciences
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- Jûtten, Peter (författare)
- Taros Chemicals GmbH & Co
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- Cerullo, Gabriella (författare)
- Department of Chemical Sciences, University of Naples "Federico II"
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- Faraco, Vincenza (författare)
- Department of Chemical Sciences, University of Naples "Federico II"
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- Papadopoulou, Adamantia (författare)
- Institute of Biosciences and Applications NCSR "Demokritos," Laboratory of Cell Proliferation and Aging
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- Kletsas, Dimitris (författare)
- Institute of Biosciences and Applications NCSR "Demokritos," Laboratory of Cell Proliferation and Aging
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- Ralli, Marianna (författare)
- Korres Natural Products
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- Rova, Ulrika (författare)
- Luleå tekniska universitet,Kemiteknik
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- Christakopoulos, Paul (författare)
- Luleå tekniska universitet,Kemiteknik
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(creator_code:org_t)
- 2017-01-11
- 2017
- Engelska.
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Ingår i: Applied Microbiology and Biotechnology. - : Springer. - 0175-7598 .- 1432-0614. ; 101:8, s. 3213-3226
- Relaterad länk:
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https://link.springe...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Five feruloyl esterases (FAEs; EC 3.1.1.73), FaeA1, FaeA2, FaeB1, and FaeB2 from Myceliophthora thermophila C1 and MtFae1a from M. thermophila ATCC 42464, were tested for their ability to catalyze the transesterification of vinyl ferulate (VFA) with prenol in detergentless microemulsions. Reaction conditions were optimized investigating parameters such as the medium composition, the substrate concentration, the enzyme load, the pH, the temperature, and agitation. FaeB2 offered the highest transesterification yield (71.5 ± 0.2%) after 24 h of incubation at 30 °C using 60 mM VFA, 1 M prenol, and 0.02 mg FAE/mL in a mixture comprising of 53.4:43.4:3.2 v/v/v n-hexane:t-butanol:100 mM MOPS-NaOH, pH 6.0. At these conditions, the competitive side hydrolysis of VFA was 4.7-fold minimized. The ability of prenyl ferulate (PFA) and its corresponding ferulic acid (FA) to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was significant and similar (IC50 423.39 μM for PFA, 329.9 μM for FA). PFA was not cytotoxic at 0.8–100 μM (IC50 220.23 μM) and reduced intracellular reactive oxygen species (ROS) in human skin fibroblasts at concentrations ranging between 4 and 20 μM as determined with the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Nyckelord
- Biokemisk processteknik
- Biochemical Process Engineering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Antonopoulou, Io
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Leonov, Laura
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Jûtten, Peter
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Cerullo, Gabriel ...
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Faraco, Vincenza
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Papadopoulou, Ad ...
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visa fler...
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Kletsas, Dimitri ...
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Ralli, Marianna
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Rova, Ulrika
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Christakopoulos, ...
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Bioprocessteknik
- Artiklar i publikationen
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Applied Microbio ...
- Av lärosätet
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Luleå tekniska universitet