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Evolution of the fe...
Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis
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- Varriale, Simona (author)
- Department of Chemical Sciences, Complesso Universitario Monte S. Angelo, University of Naples Federico II, Naples, Italy
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- Cerullo, Gabriella (author)
- Department of Chemical Sciences, Complesso Universitario Monte S. Angelo, University of Naples Federico II, Naples, Italy
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- Antonopoulou, Io, 1989- (author)
- Luleå tekniska universitet,Kemiteknik
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- Christakopoulos, Paul (author)
- Luleå tekniska universitet,Kemiteknik
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- Rova, Ulrika (author)
- Luleå tekniska universitet,Kemiteknik
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- Tron, Thierry (author)
- Centrale Marseille, CNRS, Aix Marseille Université, Marseille, France
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- Fauré, Régis (author)
- LISBP, CNRS, INRA, INSA, Université de Toulouse, Toulouse, France
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- Jütten, Peter (author)
- Taros Chemicals GmbH & Co. KG, Dortmund, Germany
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- Piechot, Alexander (author)
- Taros Chemicals GmbH & Co. KG, Dortmund, Germany
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- Brás, Joana L. A. (author)
- NzyTech LDA, Estrada Do Paco Do Lumiar Campus Do Lumiar Ed., Portugal
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- Fontes, Carlos M. G. A. (author)
- NzyTech LDA, Estrada Do Paco Do Lumiar Campus Do Lumiar Ed., Portugal
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- Faraco, Vincenza (author)
- Department of Chemical Sciences, Complesso Universitario Monte S. Angelo, University of Naples Federico II, Naples, Italy
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Department of Chemical Sciences, Complesso Universitario Monte S Angelo, University of Naples Federico II, Naples, Italy Kemiteknik (creator_code:org_t)
- 2018-04-23
- 2018
- English.
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In: Applied Microbiology and Biotechnology. - : Springer. - 0175-7598 .- 1432-0614. ; 102:12, s. 5185-5196
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The chemical syntheses currently employed for industrial purposes, including in the manufacture of cosmetics, present limitations such as unwanted side reactions and the need for harsh chemical reaction conditions. In order to overcome these drawbacks, novel enzymes are developed to catalyze the targeted bioconversions. In the present study, a methodology for the construction and the automated screening of evolved variants library of a Type B feruloyl esterase from Myceliophthora thermophila (MtFae1a) was developed and applied to generation of 30,000 mutants and their screening for selecting the variants with higher activity than the wild-type enzyme. The library was generated by error-prone PCR of mtfae1a cDNA and expressed in Saccharomyces cerevisiae. Screening for extracellular enzymatic activity towards 4-nitrocatechol-1-yl ferulate, a new substrate developed ad hoc for high-throughput assays of feruloyl esterases, led to the selection of 30 improved enzyme variants. The best four variants and the wild-type MtFae1a were investigated in docking experiments with hydroxycinnamic acid esters using a model of 3D structure of MtFae1a. These variants were also used as biocatalysts in transesterification reactions leading to different target products in detergentless microemulsions and showed enhanced synthetic activities, although the screening strategy had been based on improved hydrolytic activity.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Keyword
- Biokemisk processteknik
- Biochemical Process Engineering
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Varriale, Simona
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Cerullo, Gabriel ...
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Antonopoulou, Io ...
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Christakopoulos, ...
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Rova, Ulrika
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Tron, Thierry
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show more...
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Fauré, Régis
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Jütten, Peter
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Piechot, Alexand ...
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Brás, Joana L. A ...
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Fontes, Carlos M ...
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Faraco, Vincenza
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Bioprocess Techn ...
- Articles in the publication
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Applied Microbio ...
- By the university
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Luleå University of Technology