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Separation of nucle...
Separation of nucleoprotein complexes with antioxidant activity from yeast Saccharomyces cerevisiae
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- Butylina, Svetlana (author)
- Department of Chemical Technology, Lappeenranta University of Technology
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- Shataeva, Larisa K. (author)
- bInstitute of Macromolecular Compounds, Russian Academy of Science
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- Nyström, Marianne (author)
- Department of Chemical Technology, Lappeenranta University of Technology
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Separation and Purification Technology. - : Elsevier BV. - 1383-5866 .- 1873-3794. ; 53:1, s. 64-70
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- In this study, complexes of nucleic acids and acidic nuclear proteins were isolated from baker's yeast cells of Saccharomyces cerevisiae by mild alkaline extraction followed by precipitation with acetic acid. The optimal composition of the buffer applied for extraction of nucleoprotein complexes was determined. The high and low molar mass fractions of the nucleoprotein complexes were separated using cross-flow microfiltration through track-etched membranes. The molar masses were determined by intrinsic viscosity measurements and the molar mass distribution in nucleoprotein complexes was studied by gel permeation chromatography. The protein component of the nucleoprotein complexes was purified on a strongly basic anion exchange resin. The high molar mass nucleoprotein complexes could induce the growth of culture cells of S. cerevisiae exposed to ultraviolet irradiation or treated with hydrogen peroxide
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
Keyword
- Trä och bionanokompositer
- Wood and Bionanocomposites
Publication and Content Type
- ref (subject category)
- art (subject category)
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