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Screening of novel ...
Screening of novel feruloyl esterases from Talaromyces wortmannii for the development of efficient and sustainable syntheses of feruloyl derivatives
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- Antonopoulou, Io, 1989- (author)
- Luleå tekniska universitet,Kemiteknik
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- Iancu, Laura (author)
- Dupont Industrial Biosciences
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- Jütten, Peter (author)
- Taros Chemicals GmbH & Co KG
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- Piechot, Alexander (author)
- Taros Chemicals GmbH & Co KG
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- Rova, Ulrika (author)
- Luleå tekniska universitet,Kemiteknik
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- Christakopoulos, Paul (author)
- Luleå tekniska universitet,Kemiteknik
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(creator_code:org_t)
- Elsevier, 2019
- 2019
- English.
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In: Enzyme and microbial technology. - : Elsevier. - 0141-0229 .- 1879-0909. ; 120, s. 124-135
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The feruloyl esterases Fae125, Fae7262 and Fae68 from Talaromyces wortmannii were screened in 10 different solvent: buffer systems in terms of residual hydrolytic activity and of the ability for the transesterification of vinyl ferulate with prenol or L-arabinose. Among the tested enzymes, the acetyl xylan-related Fae125 belonging to the phylogenetic subfamily 5 showed highest yield and selectivity for both products in alkane: buffer systems (n-hexane or n-octane). Response surface methodology, based on a 5-level and 6-factor central composite design, revealed that the substrate molar ratio and the water content were the most significant variables for the bioconversion yield and selectivity. The effect of agitation, the possibility of DMSO addition and the increase of donor concentration were investigated. After optimization, competitive transesterification yields were obtained for prenyl ferulate (87.5-92.6%) and L-arabinose ferulate (56.2-61.7%) at reduced reaction times (≤ 24 h) resulting in good productivities (> 1 g/L/h, >300 kg product/kg FAE). The enzyme could be recycled for six consecutive cycles retaining 66.6% of the synthetic activity and 100% of the selectivity.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Keyword
- Biokemisk processteknik
- Biochemical Process Engineering
Publication and Content Type
- ref (subject category)
- art (subject category)
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