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Hemicellulolytic activity of Fusarium oxysporum grown on sugar beet pulp. Production of extracellular arabinanase
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- Cheilas, T (author)
- Department of Chemical Engineering, Biotechnology Laboratory, National Technical University of Athens
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- Stoupis, T (author)
- Department of Chemical Engineering, Biotechnology Laboratory, National Technical University of Athens
- Christakopoulos, Paul (author)
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- 2000
- 2000
- English.
- In: Process Biochemistry. - 1359-5113 .- 1873-3298. ; 35:6, s. 557-561
- Journal article (peer-reviewed)
Abstract
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- Fusarium oxysporum F3 exhibited hemicellulolytic enzymic activity when grown on sugar beet pulp, a by-product of the sugar industry. The growth medium was specifically optimised for enhanced production of extracellular arabinanase. The optimum medium contained sugar beet pulp (4%, w/v) and corn steep liquor (6%, v/v) as carbon and nitrogen sources, respectively. Arabinanase activity as high as 0.25 U/ml of culture was obtained, which compared favourably to those reported for other microorganisms. Optimal arabinanase activity was observed at pH 6-7 and 50 °C. Investigation of the degradation of the main components of sugar beet pulp showed that arabinose containing polysaccharides and pectin were first degraded, followed by the glucose-containing polysaccharides.
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