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Drug-Directed Morph...
Drug-Directed Morphology Changes in Polymerization-Induced Self-Assembly (PISA) Influence the Biological Behavior of Nanoparticles
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- Cao, Cheng (author)
- Centre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney 2052, Australia; Australia Nuclear Science and Technology Organisation, Lucas Heights 2234, Australia
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- Chen, Fan (author)
- Centre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney 2052, Australia
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- Garvey, Christopher J. (author)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Australia Nuclear Science and Technology Organisation, Lucas Heights 2234, Australia; Lund Institute for Advanced Neutron and X-ray Scattering, Lund 22100, Sweden
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- Stenzel, Martina H (author)
- Centre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney 2052, Australia
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(creator_code:org_t)
- 2020-06-09
- 2020
- English.
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In: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 12:27, s. 30221-30233
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The effect of the hydrophobic block length on the morphologies of polymerization-induced self-assembled (PISA) nanoparticles is well understood. However, the influence of drug loading on the phase morphology of the nanoparticles during the PISA process, and the resulting biological function of PISA nanoparticles, has barely been investigated. In this work, we show that the addition of a drug, curcumin, during the PISA process shifts the phase diagram toward different morphologies. The PISA system was based on hydrophilic poly(2-(methacryloyloxy)ethylphosphorylcholine) (PMPC), which was chain extended with hydrophobic methyl methacrylate (MMA) in various concentrations of curcumin. According to transmission electron microscopy, the presence of curcumin led to the transition of, for example, worms to polymersome and micelles to worms analysis. To understand the interaction between polymer particles and drug, small-angle X-ray scattering (SAXS), small-angle neutron scattering (SANS), and fluorescence lifetime measurements were carried out. These measurements show that curcumin is predominantly located in the core in the case of micelles and worms while it is found in the shell of polymersomes. The change in morphology influences the cellular uptake by MCF-7 cells and the movement of the particles in multicellular cancer spheroids (3D model). With the increasing amount of drug, the cellular uptake of micelles and worms was enhanced with the increasing grafting density of MPC chains, which contrasts the decreasing cellular uptake in the higher drug-loaded polymersomes due to the lower shell hydration.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
Keyword
- drug delivery
- nonspherical
- phosphorylcholine; micelles
- polymerization induced self-assembly (PISA)
- polymersomes
- scattering analysis
- self-assembly
Publication and Content Type
- ref (subject category)
- art (subject category)
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