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Bisphosphonate Liga...
Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor : Complementary Match of Supramolecular and Dynamic Chemistry
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- Bergdahl, Gizem Ertürk (author)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),CapSenze Biosystems AB, Lund, Sweden
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- Akhoundian, Maedeh (author)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV)
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- Lueg-Althoff, Kyra (author)
- Department of Chemistry, University of Duisburg-Essen, Universitätsstr. 7, Essen, 45117, Germany
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- Shinde, Sudhirkumar (author)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,School of Chemistry and Chemical Engineering, Queens University Belfast, Northern Ireland, United Kingdom
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- Yeung, Sing Yee (author)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Hedström, Martin (author)
- CapSenze Biosystems AB, Lund, 223 63, Sweden; Department of Biotechnology, Lund University, Lund, 223 62, Sweden
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- Schrader, Thomas (author)
- Department of Chemistry, University of Duisburg-Essen, Universitätsstr. 7, Essen, 45117, Germany
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- Mattiasson, Bo (author)
- CapSenze Biosystems AB, Lund, 223 63, Sweden; Department of Biotechnology, Lund University, Lund, 223 62, Sweden
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- Sellergren, Börje (author)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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(creator_code:org_t)
- Royal Society of Chemistry, 2019
- 2019
- English.
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In: New Journal of Chemistry. - : Royal Society of Chemistry. - 1144-0546 .- 1369-9261. ; 43:2, s. 847-852
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for Trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the strong interaction of bisphosphonate with lysine/arginine residues on the surface of Trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.
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- art (subject category)
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