Franz cells for facile biosensor evaluation : A case of HRP/SWCNT-based hydrogen peroxide detection via amperometric and wireless modes

• Reducing animal use in biosensor research requires broader use of in vitro methods. In this work, we present a novel application of Franz cells suitable for biosensor development and evaluation in vitro. The work describes how Franz cell can be equipped with electrodes enabling characterization of biosensors in close proximity to skin. As an example of a sensor, hydrogen peroxide biosensor was prepared based on horseradish peroxidase (HRP)/single-walled carbon nanotube (SWCNT)-modified textile. The electrode exhibited lower detection limit of 0.3 μM and sensitivity of 184 μA mM−1 cm−2. The ability of this biosensor to monitor H2O2 penetration through skin and dialysis membranes was evaluated in Franz cell setup in amperometric and wireless modes. The results also show that catalase activity present in skin is a considerable problem for epidermal sensing of H2O2. This work highlights opportunities and obstacles that can be addressed by assessment of biosensors in Franz cell setup before progressing to their testing in animals and humans.

Subject headings

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

Epidermal sensing

Franz cell

Hydrogen peroxide biosensor

Skin membrane

Animals

Biosensors

Electrodes

Oxidation

Amperometric

H$-2$/O$-2$

Horse-radish peroxidase

Hydrogen peroxide biosensors

Hydrogen peroxide detections

In-vitro

Single-walled carbon

Hydrogen peroxide

Publication and Content Type

ref (subject category)

art (subject category)