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Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy

Chushkin, Yuriy (author)
ESRF, The European Synchrotron, 71 Avenue des Martyrs, CS40220, 38043 Grenoble Cedex 9, France
Gulotta, Alessandro (author)
Division of Physical Chemistry, Lund University, Naturvetarvägen 14, 22100 Lund, Sweden
Roosen-Runge, Felix (author)
Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Division of Physical Chemistry, Lund University, Naturvetarvägen 14, 22100 Lund, Sweden
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Pal, Antara (author)
Division of Physical Chemistry, Lund University, Naturvetarvägen 14, 22100 Lund, Sweden
Stradner, Anna (author)
Division of Physical Chemistry, Lund University, Naturvetarvägen 14, 22100 Lund, Sweden; Lund Institute of advanced Neutron and X-ray Science LINXS, Lund University, Lund, Sweden
Schurtenberger, Peter (author)
Division of Physical Chemistry, Lund University, Naturvetarvägen 14, 22100 Lund, Sweden; Lund Institute of advanced Neutron and X-ray Science LINXS, Lund University, Lund, Sweden
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 (creator_code:org_t)
2022-11-29
2022
English.
In: Physical Review Letters. - : American Physical Society. - 0031-9007 .- 1079-7114. ; 129:23
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.

Subject headings

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

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