Impact of Strontium Functionalized Moderately Rough vs. Turned Titanium Disks on Biofilm Formation and Bacterial Viability

• Aim: To study the effect of Sr coated Ti disks with smooth and moderately rough surface on biofilm formation, gingipain activity, inhibition of growth of Porphyromonas gingivalis, Escherichia coli and Staphylococcus aureus using amulti-species model mimicking the subgingival environment, and to explore the pattern of Sr ion release.Materials and methods: the antimicrobial potential of Sr coated disks, i.e., smoothSr (S-Sr) and rough Sr (R-Sr) was investigated using a five species bacterial model and compared to uncoated Ti disks, i.e., smooth Ti (S-Ti) and rough Ti (RTi)at 3 different time point: 1, 3 and 6 days. Biofilm formation was investigated using LIVE/DEAD BacLight staining and confocal scanning laser microscopy and image analysis software, while inhibition of growth of P. gingivalis, E. coli and S. aureus was measured using qPCRr or growth on selective agar medium. Gingipain activity was measured using fluorescent substrate BIKKAM-16. Sr ion release from Sr coated disks was investigated up to 15 days.Results: S-Sr and R-Sr were associated with statistically significant reduction in number of viable cells compared to S-Ti and R-Ti at all different times of investigation for all multispecies tested (p < 0.001). Higher proteolytic activity associated with uncoated Ti disks compared to Sr coated disks in the P. gingivalis multispecies test at day 6. Utilizing qPCR, P. gingivalis remined undetected at all time points of evaluation for all disks, while in plate counting method, coated surfaces were associated with notable growth inhibition of both E.coil and S.aureus, using MacConkey and 110-plates, respectively. S-Sr had slightly higher release of Sr ion in the first 24 hours than R-Sr, while both Sr coated surfaces sustained stable release up to 15 days.Conclusion: In the present study, Sr coated surface, i.e., S-Sr and R-Sr, demonstrated great antimicrobial potential against multiple bacterial associated with peri-implant infection and provided a possible strategy for Ti implants infection prevention.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Odontologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dentistry (hsv//eng)

Publication and Content Type

vet (subject category)

ovr (subject category)