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The effect of lamin...
The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation
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- Schwartz Filho, Humberto Osvaldo (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
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- Bougas, Kostas (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
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Coelho, Paulo G (författare)
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Xue, Ying (författare)
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- Hayashi, Mariko (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
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Faeda, Rafael Silveira (författare)
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Marcantonio, Rosemary Adriana Chiérici (författare)
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Ono, Daisuke (författare)
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Kobayashi, Fumio (författare)
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Kamal, Mustafa (författare)
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- Wennerberg, Ann (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
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- Jimbo, Ryo (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
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(creator_code:org_t)
- Hindawi Publishing Corporation, 2012
- 2012
- Engelska.
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Ingår i: International Journal of Biomaterials. - : Hindawi Publishing Corporation. - 1687-8787 .- 1687-8795. ; 2012
- Relaterad länk:
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https://doi.org/10.1...
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https://mau.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Schwartz Filho, ...
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Bougas, Kostas
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Coelho, Paulo G
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Xue, Ying
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Hayashi, Mariko
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Faeda, Rafael Si ...
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visa fler...
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Marcantonio, Ros ...
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Ono, Daisuke
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Kobayashi, Fumio
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Kamal, Mustafa
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Wennerberg, Ann
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Jimbo, Ryo
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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och Klinisk medicin
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och Odontologi
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Malmö universitet