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Surface-dependent c...
Surface-dependent conformations of human plasma fibronectin adsorbed to silica, mica, and hydrophobic surfaces, studied with use of Atomic Force Microscopy
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- Bergkvist, Magnus (författare)
- Mälardalens högskola,Institutionen för biologi och kemiteknik,Uppsala University, Sweden
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- Carlsson, Jan (författare)
- Uppsala University, Sweden
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- Oscarsson, Sven (författare)
- Mälardalens högskola,Institutionen för biologi och kemiteknik,Uppsala University, Sweden
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(creator_code:org_t)
- 2003-01-09
- 2003
- Engelska.
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Ingår i: Journal of biomedical materials research. Part A.. - : Wiley. - 0021-9304 .- 1097-4636. ; 64:2, s. 349-356
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Human plasma fibronectin (Fn) is a large flexible protein stabilized by intermolecular ionic interactions forming a compact structure. On altering solution conditions, the structure can revert to a more expanded state, thereby exposing previously hidden domains (e.g., cell-binding sites). Electron microscopy images of Fn air-sprayed onto mica surfaces show elongated protein structures, indicating a surface-induced structural change. This makes it interesting to investigate the influence of surface properties on the structure of adsorbed Fn. We have used intermittent-contact Atomic Force Microscopy to investigate the structure of Fn adsorbed onto mica, silica, and methylated silica surfaces. We observed that on silica surfaces, which is hydrophilic, most (70%) of the molecules had an elongated structure with partial intramolecular chain interactions, compare to molecules adsorbed on hydrophobic, methylated surfaces, where a compact structure predominated (70%). On mica surfaces, both compact and elongated protein structures were observed, with a slight preference for the elongated form (53%). Results show that surface physical properties influence the molecular structure of fibronectin on adsorption, which could provide useful information in understanding surface-induced in vivo responses.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- Absorption
- Aluminum Silicates
- Fibronectins/*chemistry/ultrastructure
- Microscopy; Atomic Force
- Models; Molecular
- Protein Conformation
- Research Support; Non-U.S. Gov't
- Silicon Dioxide
- Surface Properties
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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