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Chemical transforma...
Chemical transformations in individual ultrasmall biomimetic containers
- Article/chapterEnglish1999
Publisher, publication year, extent ...
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Stanford Univ, Dept Chem, Stanford, CA 94305 USA. Univ Gothenburg, Dept Chem, S-41296 Gothenburg, Sweden. Pomona Coll, Dept Chem, Claremont, CA 91711 USA.AMER ASSOC ADVANCEMENT SCIENCE,1999
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Numbers
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LIBRIS-ID:oai:DiVA.org:mdh-51738
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https://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-51738URI
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https://doi.org/10.1126/science.283.5409.1892DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes.
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Wilson, C F
(author)
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Ryttsen, F
(author)
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Strömberg, AnetteGöteborg University, Sweden(Swepub:mdh)asg03
(author)
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Farre, C
(author)
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Karlsson, A
(author)
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Nordholm, S
(author)
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Gaggar, A
(author)
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Modi, B P
(author)
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Moscho, A
(author)
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Garza-Lopez, R A
(author)
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Orwar, O
(author)
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Zare, R N
(author)
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Göteborg University, Sweden
(creator_code:org_t)
Related titles
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In:ScienceStanford Univ, Dept Chem, Stanford, CA 94305 USA. Univ Gothenburg, Dept Chem, S-41296 Gothenburg, Sweden. Pomona Coll, Dept Chem, Claremont, CA 91711 USA. : AMER ASSOC ADVANCEMENT SCIENCE283:5409, s. 1892-18950036-80751095-9203
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Chiu, D T
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Wilson, C F
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Ryttsen, F
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Strömberg, Anett ...
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Farre, C
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Karlsson, A
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Nordholm, S
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Gaggar, A
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Modi, B P
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Moscho, A
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Garza-Lopez, R A
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Orwar, O
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Zare, R N
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Science
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Mälardalen University