Manipulating the biochemical nanoenvironment around single molecules contained within vesicles

• A method to study single-molecule reactions confined in a biomimetic container is described. The technique combines rapid vesicle preparation, optical trapping and fluorescence confocal microscopy for performing simultaneous single-vesicle trapping and single-molecule detection experiments. The collisional environment between a single enzyme and substrate inside a vesicle is characterized by a Brownian dynamics Monte Carlo simulation. (C) 1999 Elsevier Science B.V. All rights reserved.

Subject headings

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Keyword

biochemical nanoenvironment

single molecules

vesicles

Publication and Content Type

ref (subject category)

art (subject category)