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Phase behaviour of the 1-monooleoyl-rac-glycerol/n-octyl-beta-D-glucoside/water system
- Persson, Gerd (author)
- Edlund, Håkan (author)
-
- Lindblom, Göran (author)
- Umeå universitet,Kemiska institutionen
- (creator_code:org_t)
- Springer, 2004
- 2004
- English.
- In: TRENDS IN COLLOID AND INTERFACE SCIENCE XVI. - : Springer. - 3540005536 ; , s. 36-39, s. 36-9
- Conference paper (peer-reviewed)
Abstract
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- Obtaining high-quality crystals for X-ray diffraction from membrane proteins has proven to be a difficult task. One recently presented method utilises the cubic phases formed by 1-monooleoyl-rac-glycerol (MO). Removing the proteins from their native environment requires the use of surfactants. One commonly used surfactant is n-octyl-beta-D-glucopyranoside (OG). Using NMR techniques and visual observations, the ternary phase diagram of MO/OG/(H2O)-H-2 was outlined at 25 degreesC. The preliminary data show that all phases present in the binary systems at this temperature are also found in the ternary. Further, at the OG-rich side, an additional phase that appears to be hexagonal occurs. Addition of minor amounts (approximate to1.5 wt/wt %) of OG converts the cubic phases of MO to a lamellar structure, while the OG-rich cubic phase is able to dissolve about 15 wt/wt % MO. OG in water forms a large micellar solution phase;. Increasing the MO concentration at constant water content leads to a series of two- and three-phase areas in which one or two phases are in equilibrium with almost pure water.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Keyword
- MEMBRANE-PROTEIN CRYSTALLIZATION; LIPIDIC CUBIC PHASES; DIAGRAM
Publication and Content Type
- ref (subject category)
- kon (subject category)
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