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Segmented flow gene...
Segmented flow generator for serial crystallography at the European X-ray free electron laser
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-09-09
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Nature Research,2020
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:miun-41548
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https://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-41548URI
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https://doi.org/10.1038/s41467-020-18156-7DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.
Subject headings and genre
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NATURVETENSKAP Fysik Atom- och molekylfysik och optik hsv//swe
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NATURAL SCIENCES Physical Sciences Atom and Molecular Physics and Optics hsv//eng
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crystallography
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droplet
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enzyme activity
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high pressure
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laser method
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segmentation
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water treatment
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aqueous solution
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Article
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conformational transition
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controlled study
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crystal structure
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high performance liquid chromatography
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physical parameters
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pulse rate
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synchrotron radiation
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three dimensional printing
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X ray crystallography
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devices
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electron
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hydrodynamics
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lab on a chip
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laser
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ultrastructure
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2-dehydro-3-deoxyphosphooctonate aldolase
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Escherichia coli protein
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lyase
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Aldehyde-Lyases
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Electrons
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Escherichia coli Proteins
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Lab-On-A-Chip Devices
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Lasers
Added entries (persons, corporate bodies, meetings, titles ...)
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Cruz Villarreal, J.
(author)
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Messerschmidt, M.
(author)
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Kim, D.
(author)
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Coe, J. D.
(author)
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Thifault, D.
(author)
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Botha, S.
(author)
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Egatz-Gomez, A.
(author)
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Gandhi, S.
(author)
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Brehm, G.
(author)
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Conrad, C. E.
(author)
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Hansen, D. T.
(author)
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Madsen, C.
(author)
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Bajt, S.
(author)
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Meza-Aguilar, J. D.
(author)
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Oberthür, D.
(author)
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Wiedorn, M. O.
(author)
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Fleckenstein, H.
(author)
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Mendez, D.
(author)
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Knoška, J.
(author)
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Martin-Garcia, J. M.
(author)
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Hu, H.
(author)
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Lisova, S.
(author)
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Allahgholi, A.
(author)
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Gevorkov, Y.
(author)
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Ayyer, K.
(author)
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Aplin, S.
(author)
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Ginn, H. M.
(author)
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Graafsma, H.Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany(Swepub:miun)exheigra
(author)
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Morgan, A. J.
(author)
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Greiffenberg, D.
(author)
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Klujev, A.
(author)
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Laurus, T.
(author)
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Poehlsen, J.
(author)
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Trunk, U.
(author)
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Mezza, D.
(author)
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Schmidt, B.
(author)
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Kuhn, M.
(author)
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Fromme, R.
(author)
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Sztuk-Dambietz, J.
(author)
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Raab, N.
(author)
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Hauf, S.
(author)
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Silenzi, A.
(author)
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Michelat, T.
(author)
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Xu, C.
(author)
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Danilevski, C.
(author)
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Parenti, A.
(author)
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Mekinda, L.
(author)
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Weinhausen, B.
(author)
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Mills, G.
(author)
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Vagovic, P.
(author)
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Kim, Y.
(author)
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Kirkwood, H.
(author)
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Bean, R.
(author)
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Bielecki, J.
(author)
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Stern, S.
(author)
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Giewekemeyer, K.
(author)
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Round, A. R.
(author)
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Schulz, J.
(author)
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Dörner, K.
(author)
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Grant, T. D.
(author)
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Mariani, V.
(author)
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Barty, A.
(author)
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Mancuso, A. P.
(author)
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Weierstall, U.
(author)
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Spence, J. C. H.
(author)
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Chapman, H. N.
(author)
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Zatsepin, N.
(author)
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Fromme, P.
(author)
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Kirian, R. A.
(author)
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Ros, A.
(author)
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Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany
(creator_code:org_t)
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In:Nature Communications: Nature Research11:12041-1723
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Echelmeier, A.
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Cruz Villarreal, ...
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Messerschmidt, M ...
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Kim, D.
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Coe, J. D.
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Thifault, D.
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show more...
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Botha, S.
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Egatz-Gomez, A.
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Gandhi, S.
-
Brehm, G.
-
Conrad, C. E.
-
Hansen, D. T.
-
Madsen, C.
-
Bajt, S.
-
Meza-Aguilar, J. ...
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Oberthür, D.
-
Wiedorn, M. O.
-
Fleckenstein, H.
-
Mendez, D.
-
Knoška, J.
-
Martin-Garcia, J ...
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Hu, H.
-
Lisova, S.
-
Allahgholi, A.
-
Gevorkov, Y.
-
Ayyer, K.
-
Aplin, S.
-
Ginn, H. M.
-
Graafsma, H.
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Morgan, A. J.
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Greiffenberg, D.
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Klujev, A.
-
Laurus, T.
-
Poehlsen, J.
-
Trunk, U.
-
Mezza, D.
-
Schmidt, B.
-
Kuhn, M.
-
Fromme, R.
-
Sztuk-Dambietz, ...
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Raab, N.
-
Hauf, S.
-
Silenzi, A.
-
Michelat, T.
-
Xu, C.
-
Danilevski, C.
-
Parenti, A.
-
Mekinda, L.
-
Weinhausen, B.
-
Mills, G.
-
Vagovic, P.
-
Kim, Y.
-
Kirkwood, H.
-
Bean, R.
-
Bielecki, J.
-
Stern, S.
-
Giewekemeyer, K.
-
Round, A. R.
-
Schulz, J.
-
Dörner, K.
-
Grant, T. D.
-
Mariani, V.
-
Barty, A.
-
Mancuso, A. P.
-
Weierstall, U.
-
Spence, J. C. H.
-
Chapman, H. N.
-
Zatsepin, N.
-
Fromme, P.
-
Kirian, R. A.
-
Ros, A.
-
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Mid Sweden University