Dynamics of the Lipidome in a Colon Simulator

Kråkström, MatildaTurku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland (author)

Current evidence suggests that gut microbiome-derived lipids play a crucial role in the regulation of host lipid metabolism. However, not much is known about the dynamics of gut microbial lipids within the distinct gut biogeographic. Here we applied targeted and untargeted lipidomics to in vitro-derived feces. Simulated intestinal chyme was collected from in vitro gut vessels (V1-V4), representing proximal to distal parts of the colon after 24 and 48 h with/without polydextrose treatment. In total, 44 simulated chyme samples were collected from the in vitro colon simulator. Factor analysis showed that vessel and time had the strongest impact on the simulated intestinal chyme lipid profiles. We found that levels of phosphatidylcholines, sphingomyelins, triacylglycerols, and endocannabinoids were altered in at least one vessel (V1-V4) during simulation. We also found that concentrations of triacylglycerols, diacylglycerols, and endocannabinoids changed with time (24 vs. 48 h of simulation). Together, we found that the simulated intestinal chyme revealed a wide range of lipids that remained altered in different compartments of the human colon model over time.

Dickens, Alex MTurku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland (author)
Alves, Marina AmaralTurku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland (author)
Forssten, Sofia D.Global Health and Nutrition Sciences, International Flavors & Fragrances, 02460 Kantvik, Finland (author)
Ouwehand, Arthur C.Global Health and Nutrition Sciences, International Flavors & Fragrances, 02460 Kantvik, Finland (author)
Hyötyläinen, Tuulia,1971-Örebro universitet,Institutionen för naturvetenskap och teknik(Swepub:oru)tihn (author)
Oresic, Matej,1967-Örebro universitet,Institutionen för medicinska vetenskaper,Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland(Swepub:oru)moc (author)
Lamichhane, SantoshTurku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland (author)
Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandGlobal Health and Nutrition Sciences, International Flavors & Fragrances, 02460 Kantvik, Finland (creator_code:org_t)

By the author/editor: Kråkström, Matil ...; Dickens, Alex M; Alves, Marina Am ...; Forssten, Sofia ...; Ouwehand, Arthur ...; Hyötyläinen, Tuu ...; show more...; Oresic, Matej, 1 ...; Lamichhane, Sant ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Computer and Inf ...; and Bioinformatics

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