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Belimumab concentration measurements using a homologous anti-idiotype immunoassay

Loeff, Floris C. (author)
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands
Parodis, Ioannis, 1981- (author)
Örebro universitet,Institutionen för medicinska vetenskaper,Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; Department of Gastroenterology, Dermatology and Rheumatology, Karolinska University Hospital, Stockholm, Sweden; Department of Rheumatology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden
Walhelm, Tomas (author)
Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection/Rheumatology, Linköping University, Linköping, Sweden
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Jönsen, Andreas (author)
Department of Clinical Sciences Lund, Rheumatology, Lund University, Skåne University Hospital, Lund, Sweden
Nikolopoulos, Dionysis (author)
Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; Department of Gastroenterology, Dermatology and Rheumatology, Karolinska University Hospital, Stockholm, Sweden
Sjöwall, Christopher (author)
Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection/Rheumatology, Linköping University, Linköping, Sweden
Bengtsson, Anders A. (author)
Department of Clinical Sciences Lund, Rheumatology, Lund University, Skåne University Hospital, Lund, Sweden
Kos, Dorien (author)
Sanquin Reagents B.V., Amsterdam, the Netherlands
van Leeuwen, Astrid (author)
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands
van den Broek, Bryan (author)
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands
Dijk, Lisanne (author)
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands
Jeremiasse, Jorn (author)
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands
Blomjous, Birgit S. (author)
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology and immunology Center, Amsterdam University Medical Center, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands
Voskuyl, Alexandre E. (author)
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology and immunology Center, Amsterdam University Medical Center, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands
Wolbink, Gerrit Jan (author)
Amsterdam Rheumatology and immunology Center|Reade, Amsterdam, the Netherlands; Department of Immunopathology, Sanquin Research, Amsterdam, The Netherlands, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
Bultink, Irene E. M. (author)
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology and immunology Center, Amsterdam University Medical Center, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands
Rispens, Theo (author)
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands; Department of Immunopathology, Sanquin Research, Amsterdam, The Netherlands, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
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 (creator_code:org_t)
2024
2024
English.
In: JIM - Journal of Immunological Methods. - : Elsevier. - 0022-1759 .- 1872-7905.
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)

Keyword

Anti-idiotype antibodies
Belimumab
PK/ADA assays
Rabbit monoclonal antibodies

Publication and Content Type

ref (subject category)
art (subject category)

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