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Assessment of satel...
Assessment of satellite cell number and activity status in human skeletal muscle biopsies
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Mackey, A. L. (author)
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Kjaer, M. (author)
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Charifi, N. (author)
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- Henriksson, J. (author)
- Karolinska Institutet
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Bojsen-Moller, J. (author)
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Holm, L. (author)
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- Kadi, Fawzi, 1970- (author)
- Örebro universitet,Institutionen för hälsovetenskap och medicin,RISPA- MEP-RG
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(creator_code:org_t)
- 2009-08-24
- 2009
- English.
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In: Muscle and Nerve. - : John Wiley & Sons. - 0148-639X .- 1097-4598. ; 40:3, s. 455-465
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
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- The primary aim of our study was to validate the assessment of myonuclear and satellite cell number in biopsies from human skeletal muscle. We found that 25 type I and 25 type II fibers are sufficient to estimate the mean number of myonuclei per fiber. In contrast, the assessment of satellite cells improved when more fibers were included. Second, we report that small differences in counting satellite cells using CD56 and Pax7 antibodies can be attributed to the different staining profiles. Third, we provide support for the use of Ki67 in evaluating the proportion of active satellite cells. We observed very few (up to 1.3%) active satellite cells in healthy adult skeletal muscle at rest, but they increased significantly (up to 7-fold) following muscle activity. This study provides valuable tools to assess the behavior of satellite cells, both in pathological conditions and in response to physiological stimuli.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Idrottsvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Sport and Fitness Sciences (hsv//eng)
Keyword
- Active satellite cell
- Muscle fiber
- Myonucleus
- NCAM
- Pax7
- Sports
- Idrott
- Idrottsvetenskap
- Sports Science
Publication and Content Type
- ref (subject category)
- art (subject category)
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