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Identification and ...
Identification and sequence of a nifJ-like gene in Rhodospirillum rubrum : partial characterization of a mutant unaffected in nitrogen fixation
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Lindblad, A (author)
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Jansson, J (author)
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Brostedt, E (author)
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Johansson, Magnus (author)
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Hellman, U (author)
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Nordlund, S (author)
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- 2003-10-31
- 1996
- English.
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In: Molecular Microbiology. - : Wiley. - 0950-382X .- 1365-2958. ; 20:3, s. 559-568
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- A nifJ-like gene was identified in the photosynthetic purple non-sulphur bacterium Rhodospirillum rubrum. A DNA segment hybridizing to Klebsiella pneumoniae nifJ was isolated, the gene was inactivated, and a mutant strain, SNJ-1, was constructed by allele replacement. The mutation was confirmed by DNA sequencing. Northern blotting and by the lack of pyruvate oxidoreductase activity. This is the first report of a nifJ-like gene in photosynthetic bacteria. Unexpectedly, SNJ-1 was capable of nitrogen fixation, and growth was similar to the wild-type strain under all conditions investigated. Therefore, this is also the first demonstration that a nifJ homologue, when present, is not essential for nitrogen fixation in a diazotroph. The nifJ-like gene was sequenced and found to have considerable similarity to published nifJ gene sequences from other organisms. By primer extension, the initiation site for transcription was located, and a typical sigma 54 promoter sequence was identified.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Medicine
- Medicin
Publication and Content Type
- ref (subject category)
- art (subject category)
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