Combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high throughput identification of reduced susceptibility to penicillins in Neisseria meningitidis

• A segment of penA in Neisseria meningitidis strains (n = 127), including two nucleotide sites closely associated to reduced susceptibility to penicillins, was amplified and pyrosequenced. All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pen(i)-specific sites and reduced susceptibility to penicillins was identified.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Keyword

Anti-Bacterial Agents/pharmacology

Bacterial Proteins/chemistry/genetics

Base Sequence

Humans

Microbial Sensitivity Tests/methods

Molecular Sequence Data

Neisseria meningitidis/*drug effects

Penicillin Resistance

Penicillin-Binding Proteins/chemistry/genetics

Penicillins/*pharmacology

Polymerase Chain Reaction/methods

Sensitivity and Specificity

Sequence Analysis; DNA

MEDICINE

MEDICIN

Infectious diseases

Infektionssjukdomar

Infektionssjukdomar

Infectious Diseases

Medicine

Medicin

Publication and Content Type

ref (subject category)

art (subject category)