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Inhibition of 12-li...
Inhibition of 12-lipoxygenase reduces platelet activation and prevents their mitogenic function
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- Svensson Holm, Ann-Charlotte (author)
- Linköpings universitet,Avdelningen för cellbiologi,Avdelningen för läkemedelsforskning,Hälsouniversitetet
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- Grenegård, Magnus, 1963- (author)
- Linköpings universitet,Örebro universitet,Institutionen för läkarutbildning,Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Clinical Pathology and Clinical Genetics, County Council of Östergötland, Linköping, Sweden,Avdelningen för läkemedelsforskning,Hälsouniversitetet,Örebro University, Sweden
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- Ollinger, Karin (author)
- Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Klinisk patologi och klinisk genetik
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- Lindström, Eva (author)
- Linköpings universitet,Avdelningen för läkemedelsforskning,Hälsouniversitetet
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(creator_code:org_t)
- 2013-03-27
- 2014
- English.
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In: Platelets. - London, United Kingdom : Informa Healthcare. - 0953-7104 .- 1369-1635. ; 25:2, s. 111-117
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Subject headings
Close
- The aim of the present study was to investigate the role of 12-lipoxygenase (12-LOX) on platelet-induced airway smooth muscle cell (ASMC) proliferation. Co-incubation of platelets and ASMC caused platelet activation as determined by morphological changes. Simultaneously, reactive oxygen species (ROS)-generation was detected and ASMC proliferation (measured by using the MTS assay) increased significantly. Furthermore, we found that the 12-LOX inhibitors cinnamyl-3,4-dihydroxy-a-cyanocinnamate (CDC) and Baicalein prevented platelet activation in a co-cultures of platelets and ASMC. The inhibitory effect of CDC and Baicalein on platelets was also registered in a pure platelet preparation. Specifically, the 12-LOX inhibitors reduced collagen-induced platelet aggregation both in the presence and absence of external added fibrinogen. Importantly, platelet-induced ASMC proliferation and ROS production generated during the platelet/ASMC interaction was significantly inhibited in the presence of 12-LOX inhibitors. In conclusion, our findings reveal that 12-LOX is crucial for the observed enhancement of ASMC proliferation in co-cultures of platelets and ASMC. The present result suggests that 12-LOX activity is important in the initial step of platelet/ASMC interaction and platelet activation. Such action of 12-LOX represents a potential important mechanism that may contribute to platelet-induced airway remodelling.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- 12-lipoxygenase
- airway remodelling
- airway smooth muscle
- platelet-induced proliferation
- Cellforskning
- Cell Research
- Medicine
- Medicin
Publication and Content Type
- ref (subject category)
- art (subject category)
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