Search: onr:"swepub:oai:DiVA.org:oru-37412" >
Determination of ch...
-
Ekblad, Alf,1957-University of Umeå, Umeå, Sweden
(author)
Determination of chitin in fungi and mycorrhizal roots by an improved HPLC analysis of glucosamine
- Article/chapterEnglish1996
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:oru-37412
-
https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-37412URI
-
https://doi.org/10.1007/BF00011160DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
A method to measure chitin content in fungi and ectomycorrhizal roots with high-performance liquid chromatography (HPLC) was developed. Measurements of fluorescence of 9-fluorenylmethylchloroformate (FMOC-CI) derivatives of glucosamine were made on acid hydrolysates of pure chitin, chitin-root mixtures and fungal-root mixtures. The method was applied on 5 isolates of ectomycorrhizal fungi, and ectomycorrhizal and non-mycorrhizal Pinus sylvestris roots. Interference from amino acids was removed by pre-treatment of samples with 0.2 N NaOH. This pre-treatment did not reduce the recovery of chitin, nor did plant material affect the recovery of chitin. The HPLC method was compared with a colorimetric chitin-method by measurements on root-fungal mixtures, with known fungal content. The HPLC method gave estimates of fungal biomass which were equal to the expected while the colorimetric method showed values significantly (p<0.001) lower than the expected. The present chitin method offers a sensitive and specific tool for the quantification of chitin in fungi and in ectomycorrhizal roots.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Näsholm, TorgnySwedish University of Agricultural Sciences, Umeå, Sweden
(author)
-
University of Umeå, Umeå, SwedenSwedish University of Agricultural Sciences, Umeå, Sweden
(creator_code:org_t)
Related titles
-
In:Plant and Soil178:1, s. 29-350032-079X1573-5036
Internet link
Find in a library
To the university's database