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Unlocking pathology...
Unlocking pathology archives for microRNA-profiling
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- Hoefig, Kai P (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Thorns, Christoph (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Roehle, Anja (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Kaehler, Christian (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Wesche, Kai O (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Repsilber, Dirk, 1971- (author)
- Biomathematics / Bioinformatics Group, Research Institute for the Biology of Farm Animals FBN, Dummerstorf, Germany
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- Branke, Biggi (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Thiere, Marlen (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Feller, Alfred C (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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- Merz, Hartmut (author)
- Institute for Pathology, UKSH Campus Luebeck, Luebeck
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(creator_code:org_t)
- Athens, Greece : International Institute of Anticancer Research, 2008
- 2008
- English.
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In: Anticancer Research. - Athens, Greece : International Institute of Anticancer Research. - 0250-7005 .- 1791-7530. ; 28:1A, s. 119-123
- Related links:
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https://ar.iiarjourn...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Background: MicroRNAs (miRNAs) are approximately 22 nucleotide long, non-coding RNAs that regulate gene expression by binding to the 3'-untranslated region of target mRNAs and also a variety of cellular processes. It has recently been established that dysregulation of miRNA expression can be detected in the majority of human cancers. A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material.Materials and methods: The miRNA expression profiles of freshly frozen (CRYO) and FFPE tissues of seven tonsil and four liver samples were compared, using a qPCR-based assay, profiling 157 miRNA species.Results: The significance of miRNA-profiles was barely influenced by FFPE treatment in both tissues and the variance induced by FFPE treatment was much smaller than the variance caused by biologically based differential expression.Conclusion: FFPE material is well suited for miRNA profiling.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- miRNA
- FFPE
- expression profiling
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Hoefig, Kai P
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Thorns, Christop ...
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Roehle, Anja
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Kaehler, Christi ...
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Wesche, Kai O
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Repsilber, Dirk, ...
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show more...
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Branke, Biggi
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Thiere, Marlen
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Feller, Alfred C
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Merz, Hartmut
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
- Articles in the publication
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Anticancer Resea ...
- By the university
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Örebro University