A Pisum sativum glyoxysomal malate dehydrogenase induced by cadmium exposure

• The glyoxysomal malate dehydrogenase (gMDH) catalyses the formation of oxaloacetate from malate during beta-oxidation of fatty acids in the glyoxysome. A partial Pisum sativum L. (cv. Greenfeast) cDNA was first isolated from a suppression subtractive hybridisation cDNA library obtained from heavy metal stressed plants. The full length cDNA was then isolated by rapid amplification of cDNA ends. The translated sequence showed strong similarity to Cucumis sativus and Citrullus lanatus gMDH including a typical glyoxysome-targeting presequence comprising the PTS2 motif and a cleavage site for a cystein-directed protease. Exposure of pea plants to Cd2+ induced expression of the gMDH gene in mature pea leaves indicating that the enzyme is under environmental control in addition to the normal developmental regulation pattern.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Genetik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Genetics (hsv//eng)

Keyword

Amino Acid Sequence

Base Sequence

Blotting; Northern

Cadmium/*toxicity

DNA Primers

DNA; Complementary/genetics

Enzyme Induction/drug effects

Glyoxysomes/*enzymology

Malate Dehydrogenase/*biosynthesis/*genetics

Molecular Sequence Data

Nucleic Acid Amplification Techniques

Peas/enzymology/*genetics

Sequence Analysis; DNA

Sequence Homology

Biochemistry

Biokemi

Biochemistry

Biokemi

suppression subtractive hybridization

gene-expression

b-radiation

plants

metabolism

vulgaris

cloning

cdna

cd2

Publication and Content Type

ref (subject category)

art (subject category)