Search: onr:"swepub:oai:DiVA.org:oru-42401" > A novel role for ph...
Fältnamn | Indikatorer | Metadata |
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000 | 05630naa a2200553 4500 | |
001 | oai:DiVA.org:oru-42401 | |
003 | SwePub | |
008 | 150204s2012 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:liu-80685 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-424012 URI |
024 | 7 | a https://doi.org/10.1016/j.cellsig.2012.04.0182 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-806852 URI |
040 | a (SwePub)orud (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Elvers, Margittau Medizinische Klinik III, Dept. of Cardiology and Cardiovascular Diseases, Eberhard Karls University, Tübingen, Germany4 aut |
245 | 1 0 | a A novel role for phospholipase D as an endogenous negative regulator of platelet sensitivity |
264 | 1 | a New York, USA :b Elsevier,c 2012 |
338 | a print2 rdacarrier | |
520 | a Platelet aggregation, secretion and thrombus formation play a critical role in primary hemostasis to prevent excessive blood loss. On the other hand, uncontrolled platelet activation leads to pathological thrombus formation resulting in myocardial infarction or stroke. Stimulation of heterotrimeric G-proteins by soluble agonists or immunoreceptor tyrosine based activation motif-coupled receptors that interact with immobilized ligands such as the collagen receptor glycoprotein (GP) VI lead to the activation of phospholipases that cleave membrane phospholipids to generate soluble second messengers. Platelets contain the phospholipases (PL) D1 and D2 which catalyze the hydrolysis of phosphatidylcholine to generate the second messenger phosphatidic acid (PA). The production of PA is abrogated by primary alcohols that have been widely used for the analysis of PLD-mediated processes. However, it is not clear if primary alcohols effectively reduce PA generation or if they induce PLD-independent cellular effects. In the present study we made use of the specific PLD inhibitor 5-fluoro-2-indolyl des-chlorohalopemide (FIPI) and show for the first time, that FIPI enhances platelet dense granule secretion and aggregation of human platelets. Further, FIPI has no effect on cytosolic Ca(2+) activity but needs proper Rho kinase signaling to mediate FIPI-induced effects on platelet activation. Upon FIPI treatment the phosphorylation of the PKC substrate pleckstrin was prominently enhanced suggesting that FIPI affects PKC-mediated secretion and aggregation in platelets. Similar effects of FIPI were observed in platelets from mouse wild-type and Pld1(-/-) mice pointing to a new role for PLD2 as a negative regulator of platelet sensitivity. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a PLD | |
653 | a platelets | |
653 | a secretion | |
653 | a aggregation | |
653 | a regulation | |
653 | a MEDICINE | |
700 | 1 | a Grenegård, Magnus,d 1963-u Linköpings universitet,Farmakologi,Hälsouniversitetet4 aut0 (Swepub:liu)maggr06 |
700 | 1 | a Khoshjabinzadeh, Haniehu Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut |
700 | 1 | a Münzer, Patricku Department of Physiology, Eberhard Karls University, Tübingen, Germany4 aut |
700 | 1 | a Borst, Oliveru Medizinische Klinik III, Dept. of Cardiology and Cardiovascular Diseases, Eberhard Karls University, Tübingen, Germany; Department of Physiology, Eberhard Karls University, Tübingen, Germany4 aut |
700 | 1 | a Tian, Huasongu Department of Pathology and Cell Biology, Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Medical Center, New York, USA,Columbia University, NY 10032 USA4 aut |
700 | 1 | a Di Paolo, Gilbertu Department of Pathology and Cell Biology, Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Medical Center, New York, USA,Columbia University, NY 10032 USA4 aut |
700 | 1 | a Lang, Florianu Department of Physiology, Eberhard Karls University, Tübingen, Germany4 aut |
700 | 1 | a Gawaz, Meinradu Medizinische Klinik III, Dept. of Cardiology and Cardiovascular Diseases, Eberhard Karls University, Tübingen, Germany4 aut |
700 | 1 | a Lindahl, Tomasu Östergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)tomli13 |
700 | 1 | a Fälker, Knutu Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet4 aut0 (Swepub:liu)knufa08 |
710 | 2 | a Medizinische Klinik III, Dept. of Cardiology and Cardiovascular Diseases, Eberhard Karls University, Tübingen, Germanyb Farmakologi4 org |
773 | 0 | t Cellular Signallingd New York, USA : Elsevierg 24:9, s. 1743-52q 24:9<1743-52x 0898-6568x 1873-3913 |
856 | 4 | u http://liu.diva-portal.org/smash/get/diva2:547904/FULLTEXT01 |
856 | 4 | u https://liu.diva-portal.org/smash/get/diva2:547904/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-42401 |
856 | 4 8 | u https://doi.org/10.1016/j.cellsig.2012.04.018 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-80685 |
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