Sökning: onr:"swepub:oai:DiVA.org:oru-46829" >
A comparison betwee...
A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food
-
- Thisted Lambertz, Susanne (författare)
- National Food Administration, Uppsala, Sweden
-
- Ballagi-Pordany, A. (författare)
- National Food Administration, Uppsala, Sweden
-
- Nilsson, A. (författare)
- National Food Administration, Uppsala, Sweden
-
visa fler...
-
- Norberg, P. (författare)
- National Food Administration, Uppsala, Sweden
-
- Danielsson-Tham, Marie-Louise, 1947- (författare)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2008-03-11
- 1996
- Engelska.
-
Ingår i: Journal of Applied Bacteriology. - : Blackwell Publishing. - 0021-8847. ; 81:3, s. 303-308
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The aim of this study was to develop a polymerase chain reaction (PCR) method for the detection of pathogenic Yersinia enterocolitica and to compare it with an official culture method (NMKL-117). Primers were selected for nested PCR directed at the attachment invasion locus, ail, on the bacterial chromosome, as well as at a sequence on the pathogenic marker plasmid, termed virulence factor, virF. The final results obtained by the two methods were similar. However, while the conventional method yielded contradictory data for some steps the PCR method provided unambiguous results. Considerable advantages, i.e. higher sensitivity and specificity of the PCR method, compared with the conventional method for detecting pathogenic Y. enterocolitica, were demonstrated in this study.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Nyckelord
- Måltidskunskap
- Culinary Arts and Meal Science
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas