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Gene Expression Ana...
Gene Expression Analysis of Fibroblasts from Patients with Bipolar Disorder
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- Logotheti, Marianthi (author)
- Örebro universitet,Institutionen för hälsovetenskap och medicin,Neuropsychiatric Research Laboratory, Faculty of Medicine and Health, Örebro University, Örebro, Sweden; Metabolic Engineering and Bioinformatics Group, National Hellenic Research Foundation, Athens, Greece; Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, Athens, Greece,Experimentell neuropsykiatri
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- Papadodima, Olga (author)
- Metabolic Engineering and Bioinformatics Group, Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, Greece
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- Chatziioannou, Aristotelis (author)
- Metabolic Engineering and Bioinformatics Group, Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, Greece
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- Venizelos, Nikolaos, 1946- (author)
- Örebro universitet,Institutionen för hälsovetenskap och medicin,Neuropsychiatric Research Laboratory, Faculty of Medicine and Health, Örebro University, Örebro, Sweden,Experimentell neuropsykiatri
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- Kolisis, Fragiskos (author)
- Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
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(creator_code:org_t)
- 2016
- 2015
- English.
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In: Journal of Neuropsychopharmacology & Mental Health. - : OMICS International. - 2472-095X. ; 1:1, s. 1-9
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https://oru.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Bipolar disorder is a severe, lifelong psychiatric disease. The main underlying pathophysiology of the disease is still incomprehensible. Various studies have suggested that many genes of small impact in combination with environmental factors contribute to the expression of the disease. In this study comparative transcriptomic profiling to characterize skin fibroblasts’ gene expression of bipolar disorder patients compared to healthy controls has been performed. Skin fibroblast cells from bipolar disorder patients (n=10) and marched healthy controls (n=5) have been cultured. RNA was extracted and then hybridized onto Illumina Human HT-12 v4 Expression BeadChips. Differentially expressed genes between bipolar disorder samples and healthy controls were identified by performing unequal t-test on log 2 transformed expression values. The resulting gene list was obtained by setting the p-value threshold to 0.05 and by removing genes that presented a fold change ≥ |0.5| (in log 2 scale). We concluded to 457 differentially expressed genes. Among them 127 showed an upregulation and 330 were downregulated. Τhe expression alterations of selected genes were validated by quantitative real-time polymerase chain reaction. In order to derive better insight into the biological mechanisms related to the differentially expressed genes, the lists of significant genes were subjected to pathway analysis and target prioritization indicating various processes such as calcium ion homeostasis, positive regulation of apoptotic process and cellular response to retinoic acid.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Psykiatri (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Psychiatry (hsv//eng)
Keyword
- Skin fibroblasts
- Bbipolar disorder
- transcriptome
- psychiatric diseases
- pathway analysis
- microarrays
- Psychiatry
- Psykiatri
- Molekylär medicin (genetik och patologi)
- Molecular Medicine (Genetics and Pathology)
- Biomedicin
- Biomedicine
Publication and Content Type
- ref (subject category)
- art (subject category)
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