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Liquid chromatograp...
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues
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- Nygren, Heli (author)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Seppänen-Laakso, Tuulikki (author)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Castillo, Sandra (author)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Hyötyläinen, Tuulia, 1971- (author)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Oresic, Matej, 1967- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,VTT Technical Research Centre of Finland, Espoo, Finland
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(creator_code:org_t)
- 2010-12-17
- 2011
- English.
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In: Methods in Molecular Biology. - Totowa, NJ : Humana Press. - 1064-3745 .- 1940-6029. ; 708, s. 247-257
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS(n) mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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