Gut barrier dysfunction: a primary defect in twins with Crohn's disease predominantly caused by genetic predisposition

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:oru-66791" > Gut barrier dysfunc...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Keita, ÅsaLinköpings universitet,Avdelningen för Kirurgi, Ortopedi och Onkologi,Medicinska fakulteten (author)

Gut barrier dysfunction : a primary defect in twins with Crohn's disease predominantly caused by genetic predisposition

Article/chapterEnglish2018

Publisher, publication year, extent ...

2018-04-12
Elsevier,2018
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:oru-66791
https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-66791URI
https://doi.org/10.1093/ecco-jcc/jjy045DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-154129URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Funding Agency:Region Östergötland, ALF research funds of Linköping University
Funding Agencies|Swedish Research Council-Medicine [VR-MH 2014-02537, 521-2011-2764]; Region Ostergotland, ALF research funds of Linkoping University
Background and aims: The aetiology of Crohn's disease is poorly understood. By investigating twin pairs discordant for Crohn's disease we aimed to assess if the dysregulated barrier represents a cause or a consequence of inflammation and to evaluate the impact of genetic predisposition on barrier function.Methods: Ileal biopsies from 15 twin pairs discordant for Crohn's disease (monozygotic n=9, dizygotic n=6) and 10 external controls were mounted in Ussing chambers to assess paracellular permeability to51Chromium (Cr)-EDTA and trancellular passage to non-pathogenic E. coli K-12. Experiments were performed with and without provocation with acetylsalicylic acid. Immunofluorescence and ELISA were used to quantify the expression level of tight junction proteins.Results: Healthy co-twins and affected twins displayed increased 51Cr-EDTA permeability at 120 min both with Acetylsalicylic acid (p<0.001) and without (p<0.001) when compared to controls. A significant increase in 51Cr-EDTA flux was seen already at 20 minutes in healthy monozygotic co-twins compared to controls (p≤0.05) when stratified by zygosity, but not in healthy dizygotic co-twins. No difference in E. coli passage was observed between groups. Immunofluorescence of the tight junction proteins claudin-5 and tricellulin showed lower levels in healthy co-twins (p<0.05) and affected twins (p<0.05) compared to external controls, while ELISA only showed lower tricellulin in Crohn's disease twins (p<0.05).Conclusion: Our results suggest that barrier dysfunction is a primary defect in Crohn's disease, since changes were predominantly seen in healthy monozygotic co-twins. Passage of E. coli seems to be a consequence of inflammation rather than representing a primary defect.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Gastroenterologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Gastroenterology and Hepatology hsv//eng
Crohn's disease
barrier function
genetics

Added entries (persons, corporate bodies, meetings, titles ...)

Lindqvist, Carl Mårten,1979-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Medical Sciences,Örebro University, Örebro, Sweden(Swepub:oru)melt (author)
Öst, ÅkeDepartment of Pathology and Cytology, Aleris Medilab, Täby, Sweden (author)
Ley Magana, Carlos DanielLinköpings universitet,Avdelningen för kliniska vetenskaper,Medicinska fakulteten(Swepub:liu)carle20 (author)
Schoultz, Ida,1979-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Medical Sciences,Örebro University, Örebro, Sweden(Swepub:oru)isz (author)
Halfvarson, Jonas,1970-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Gastroenterology,Örebro University, Örebro, Sweden(Swepub:oru)jshn (author)
Linköpings universitetAvdelningen för Kirurgi, Ortopedi och Onkologi (creator_code:org_t)

Related titles

In:Journal of Crohn's & Colitis: Elsevier12:10, s. 1200-12091873-99461876-4479

Internet link

Find in a library

Journal of Crohn's & Colitis (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Keita, Åsa; Lindqvist, Carl ...; Öst, Åke; Ley Magana, Carl ...; Schoultz, Ida, 1 ...; Halfvarson, Jona ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Gastroenterology ...

Articles in the publication: Journal of Crohn ...

By the university: Örebro University; Linköping University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se