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Flow Cytometry Assa...
Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
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- Potrzebowska, Katarzyna (author)
- Department of Experimental Medical Science, Lund University, Lund, Sweden
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- Lehtonen, Janne (author)
- Department of Experimental Medical Science, Lund University, Lund, Sweden
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- Samuelsson, Malin (author)
- Department of Experimental Medical Science, Lund University, Lund, Sweden
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- Svensson, Lena, 1973- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Experimental Medical Science, Lund University, Lund, Sweden
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(creator_code:org_t)
- Bio-Protocol, 2018
- 2018
- English.
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In: Bio-protocol. - : Bio-Protocol. - 2331-8325. ; 8:23
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Subject headings
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- To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow cytometry-based assay that allows the quantification of LFA-1 internalization and its re-expression on the cell surface in T lymphocytes. An advantage of using flow cytometry-based assay over biochemical methods is the low number of needed cells. This protocol can be also used to measure recycling of other receptors.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Flow cytometry
- Receptor
- LFA-1
- Endocytosis
- Exocytosis
Publication and Content Type
- ref (subject category)
- art (subject category)
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