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Omega-3 fatty acids...
Omega-3 fatty acids enhance phagocytosis of Alzheimer's disease-related amyloid-β42 by human microglia and decrease inflammatory markers
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- Hjorth, Erik (author)
- Karolinska Institutet
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- Zhu, Mingqin (author)
- Division of Neurodegeneration, Department of Neurobiology, Care Sciences & Society, Karolinska Institutet, Stockholm, Sweden
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- Toro, Veronica Cortés (author)
- Division of Neurodegeneration, Department of Neurobiology, Care Sciences & Society, Karolinska Institutet, Stockholm, Sweden
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- Vedin, Inger (author)
- Karolinska Institutet
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- Palmblad, Jan (author)
- Karolinska Institutet
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- Cederholm, Tommy (author)
- Uppsala universitet,Karolinska Institutet,Klinisk nutrition och metabolism
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- Freund-Levi, Yvonne, 1956- (author)
- Clinical Geriatrics, Department of Neurobiology, Care Sciences & Society, Karolinska Institutet, Stockholm, Sweden
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- Faxen-Irving, Gerd (author)
- Karolinska Institutet
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- Wahlund, Lars-Olof (author)
- Karolinska Institutet
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- Basun, Hans (author)
- Uppsala universitet,Geriatrik
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- Eriksdotter, Maria (author)
- Karolinska Institutet
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- Schultzberg, Marianne (author)
- Karolinska Institutet
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(creator_code:org_t)
- IOS Press, 2013
- 2013
- English.
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In: Journal of Alzheimer's Disease. - : IOS Press. - 1387-2877 .- 1875-8908. ; 35:4, s. 697-713
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Subject headings
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- The use of supplements with omega-3 (ω3) fatty acids (FAs) such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) is widespread due to proposed beneficial effects on the nervous and cardiovascular systems. Many effects of ω3 FAs are believed to be caused by down-regulation and resolution of inflammation. Alzheimer's disease (AD) is associated with inflammation mediated by microglia and astrocytes, and ω3 FAs have been proposed as potential treatments for AD. The focus of the present study is on the effects of DHA and EPA on microglial phagocytosis of the AD pathogen amyloid-β (Aβ), on secreted and cellular markers of immune activity, and on production of brain-derived neurotrophic factor (BDNF). Human CHME3 microglial cells were exposed to DHA or EPA, with or without the presence of Aβ42. Phagocytosis of Aβ42 was analyzed by flow cytometry in conjunction with immunocytochemistry using antibodies to cellular proteins. Secreted proteins were analyzed by ELISA. Both DHA and EPA were found to stimulate microglial phagocytosis of Aβ42. Phagocytosis of Aβ42 was performed by microglia with a predominance of M2 markers. EPA increased the levels of BDNF in the culture medium. The levels of TNF-α were decreased by DHA. Both DHA and EPA decreased the pro-inflammatory M1 markers CD40 and CD86, and DHA had a stimulatory effect on the anti-inflammatory M2 marker CD206. DHA and EPA can be beneficial in AD by enhancing removal of Aβ42, increasing neurotrophin production, decreasing pro-inflammatory cytokine production, and by inducing a shift in phenotype away from pro-inflammatory M1 activation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- Amyloid
- brain-derived neurotrophic factor
- cytokine
- DHA
- EPA
- interleukin
- M1
- M2
- resolution
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Hjorth, Erik
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Zhu, Mingqin
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Toro, Veronica C ...
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Vedin, Inger
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Palmblad, Jan
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Cederholm, Tommy
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Freund-Levi, Yvo ...
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Faxen-Irving, Ge ...
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Wahlund, Lars-Ol ...
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Basun, Hans
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Eriksdotter, Mar ...
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Schultzberg, Mar ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Neurology
- Articles in the publication
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Journal of Alzhe ...
- By the university
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Örebro University
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Karolinska Institutet
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Uppsala University