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Vitamin D enhances ...
Vitamin D enhances IL-1β secretion and restricts growth of Mycobacterium tuberculosis in macrophages from TB patients
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- Eklund, Daniel, 1984- (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Persson, Hans Lennart (author)
- Östergötlands Läns Landsting,Linköpings universitet,Lungmedicin,Hälsouniversitetet,Lungmedicinska kliniken US
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- Larsson, Marie C. (author)
- Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet
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- Welin, Amanda (author)
- Gothenburg University,Göteborgs universitet,Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Idh, Jonna (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Paues, Jakob (author)
- Linköpings universitet,Infektionsmedicin,Hälsouniversitetet
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- Fransson, Sven-Göran (author)
- Östergötlands Läns Landsting,Linköpings universitet,Medicinsk radiologi,Hälsouniversitetet,Röntgenkliniken i Linköping
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- Stendahl, Olle (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Schön, Thomas (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Lerm, Maria (author)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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(creator_code:org_t)
- Netherlands : Wolters Kluwer, 2013
- 2013
- English.
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In: International journal of mycobacteriology. - Netherlands : Wolters Kluwer. - 2212-5531. ; 2:1, s. 18-25
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Abstract
Subject headings
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- The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB), the bacterium responsible for tuberculosis (TB), has rekindled the interest in the role of nutritional supplementation of micronutrients, such as vitamin D, as adjuvant treatment. Here, the growth of virulent MTB in macrophages obtained from the peripheral blood of patients with and without TB was studied. The H37Rv strain genetically modified to express Vibrio harveyi luciferase was used to determine the growth of MTB by luminometry in the human monocyte-derived macrophages (hMDMs) from study subjects. Determination of cytokine levels in culture supernatants was performed using a flow cytometry-based bead array technique. No differences in intracellular growth of MTB were observed between the different study groups. However, stimulation with 100nM 1,25-dihydroxyvitamin D significantly enhanced the capacity of hMDMs isolated from TB patients to control the infection. This effect was not observed in hMDMs from the other groups. The interleukin (IL)-1β and IL-10 release by hMDMs was clearly increased upon stimulation with 1,25-dihydroxyvitamin D. Furthermore, the 1,25-dihydroxyvitamin D stimulation also led to elevated levels of TNF-α (tumor necrosis factor-alpha) and IL-12p40. It was concluded that vitamin D triggers an inflammatory response in human macrophages with enhanced secretion of cytokines, as well as enhancing the capacity of hMDMs from patients with active TB to restrict mycobacterial growth.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Human macrophages
- IL-1β
- Intracellular growth
- TB patients
- Vitamin D
- Vitamin D
- Human macrophages
- Intracellular growth
- TB patients
- IL-1β
Publication and Content Type
- ref (subject category)
- art (subject category)
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Eklund, Daniel, ...
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Persson, Hans Le ...
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Larsson, Marie C ...
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Welin, Amanda
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Idh, Jonna
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Paues, Jakob
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Fransson, Sven-G ...
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Stendahl, Olle
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Schön, Thomas
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Lerm, Maria
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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Örebro University
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Linköping University
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University of Gothenburg