onr:"swepub:oai:DiVA.org:oru-80916"
Search: onr:"swepub:oai:DiVA.org:oru-80916" > Reproducible loss o...
- 1 of 1
- Previous record
- Next record
- To hitlist
Reproducible loss of CA1 neurons following carotid artery occlusion combined with halothane-induced hypotension
-
- Bendel, Olof (author)
- Section of Clinical CNS Research, Department of Clinical Neuroscience, Karolinska Institutet, Karolinska University Hospital, Solna, Stockholm, Sweden
-
- Alkass, Kanar (author)
- Karolinska Institutet
-
- Bueters, Tjerk (author)
- Section of Clinical CNS Research, Department of Clinical Neuroscience, Karolinska Institutet, Karolinska University Hospital, Solna, Stockholm, Sweden
- show more...
- show less...
- (creator_code:org_t)
- Elsevier, 2005
- 2005
- English.
- In: Brain Research. - : Elsevier. - 0006-8993 .- 1872-6240. ; 1033:2, s. 135-142
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The 2-vessel occlusion approach to produce global ischemia in rats requires concomitant reduction of systemic blood pressure. We have utilized the hypotensive effect of halothane administrated by artificial respiration to prevent respiratory arrest and to ensure stable physiological conditions. Systemic blood pressure was reduced to 40-45 mmHg by instant adjustments of the halothane concentration. Bilateral occlusion of the carotid arteries caused a profound and reproducible ischemia, as analyzed by laser-Doppler flowmetry. In the rats exposed to 11, 12, or 13 min of ischemia, 5% died and 5% developed seizures. The extent of neuronal death in CA1 was highly correlated to the duration of ischemia. Following 11 min of ischemia, CA1 neuronal cell death, as analyzed by Fluoro-Jade, was absent 1 day after injury, variable at day 4, and consistent at day 7. The numbers of cresyl violet- and NeuN-positive neurons at day 7 were 8% and 20% of control, respectively. OX42 immunoreactivity was low and variable at day 4, but pronounced at day 7. In conclusion, this rat global ischemia model is relatively simple to perform, has a low mortality, and produces a profound and highly reproducible delayed cell death of hippocampal CA1 neurons.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Halothane
- Global ischemia
- Delayed neuronal death
- CA1
- Rat
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Brain Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Bendel, Olof
- Alkass, Kanar
- Bueters, Tjerk
- von Euler, Mia, ...
- von Euler, Gabri ...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Brain Research
- By the university
- Örebro University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
