onr:"swepub:oai:DiVA.org:oru-82849"
Search: onr:"swepub:oai:DiVA.org:oru-82849" > Flow Cytometric Mea...
- 1 of 1
- Previous record
- Next record
- To hitlist
Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
-
- Löf, Liza (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
-
- Arngården, Linda, 1984- (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
-
- Olsson-Strömberg, Ulla (author)
- Uppsala universitet,Hematologi
- show more...
- show less...
- (creator_code:org_t)
- 2017-04-04
- 2017
- English.
- In: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 7:1
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Chronic myeloid leukemia (CML) is characterized in the majority of cases by a t(9;22)(q34;q11) translocation, also called the Philadelphia chromosome, giving rise to the BCR-ABL1 fusion protein. Current treatment with tyrosine kinase inhibitors is directed against the constitutively active ABL1 domain of the fusion protein, and minimal residual disease (MRD) after therapy is monitored by real-time quantitative PCR (RQ-PCR) of the fusion transcript. Here, we describe a novel approach to detect and enumerate cells positive for the BCR-ABL1 fusion protein by combining the in situ proximity ligation assay with flow cytometry as readout (PLA-flow). By targeting of the BCR and ABL1 parts of the fusion protein with one antibody each, and creating strong fluorescent signals through rolling circle amplification, PLA-flow allowed sensitive detection of cells positive for the BCR-ABL1 fusion at frequencies as low as one in 10,000. Importantly, the flow cytometric results correlated strongly to those of RQ-PCR, both in diagnostic testing and for MRD measurements over time. In summary, we believe this flow cytometry-based method can serve as an attractive approach for routine measurement of cells harboring BCR-ABL1 fusions, also allowing simultaneously assessment of other cell surface markers as well as sensitive longitudinal follow-up.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Scientific Reports (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Löf, Liza
- Arngården, Linda ...
- Olsson-Strömberg ...
- Siart, Benjamin
- Jansson, Mattias
- Dahlin, Joakim S ...
- show more...
- Thörn, Ingrid, 1 ...
- Christiansson, L ...
- Hermansson, Moni ...
- Larsson, Anders
- Ahlstrand, Erik, ...
- Wålinder, Göran
- Söderberg, Ola, ...
- Rosenquist, Rich ...
- Landegren, Ulf
- Kamali-Moghaddam ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Hematology
- Articles in the publication
- Scientific Repor ...
- By the university
- Örebro University
- Karolinska Institutet
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
