Search: onr:"swepub:oai:DiVA.org:oru-92515" >
Elevated F-EDN corr...
Elevated F-EDN correlates with mucosal eosinophil degranulation in patients with IBS : A possible association with microbiota?
-
- Casado-Bedmar, Maite (author)
- Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden,Linköping Univ, Dept Biomed & Clin Sci, Linköping, Sweden.
-
- Meira de Faria, Felipe (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
-
- Biskou, Olga (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten
-
show more...
-
- Lindqvist, Carl Mårten, 1979- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Medical Sciences,Örebro Univ, Dept Med Sci, Örebro, Sweden.
-
- Ranasinghe, Purnika Damindi (author)
- Institute of Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, UK,Queens Univ Belfast, Sch Biol Sci, Inst Global Food Secur, Belfast, Antrim, North Ireland.
-
- Bednarska, Olga (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Region Östergötland, Mag- tarmmedicinska kliniken
-
- Peterson, Christer (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Institutionen för kvinnors och barns hälsa,Uppsala Univ, Sweden; Diagnost Dev, Sweden
-
- Walter, Susanna (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Region Östergötland, Mag- tarmmedicinska kliniken
-
- Carlsson, Maria E., 1958- (author)
- Uppsala universitet,Vårdvetenskap,Livsstil och rehabilitering vid långvarig sjukdom,Uppsala Univ, Sweden
-
- Keita, Åsa (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten
-
- Casado Bedmar, Maria Teresa (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten
-
show less...
-
Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden Linköping Univ, Dept Biomed & Clin Sci, Linköping, Sweden (creator_code:org_t)
- Alan R. Liss Inc. 2022
- 2022
- English.
-
In: Journal of Leukocyte Biology. - : Alan R. Liss Inc.. - 0741-5400 .- 1938-3673. ; 111:3, s. 655-665
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Eosinophils have been linked to functional dyspepsia; however, less is known about their role in irritable bowel syndrome (IBS). This study tested the hypothesis of alterations in levels of fecal eosinophil-derived neurotoxin (F-EDN) and eosinophil density and degranulation within the colonic mucosa of IBS patients compared with healthy controls (HC). Colonic biopsies were collected from 37 IBS patients and 20 HC and analyzed for eosinophil numbers and local degranulation of eosinophil cationic protein (ECP) by histologic procedures. Fecal samples were collected for F-EDN and microbiota analysis. Differentiated 15HL-60 cells were used in vitro to investigate the direct effect of live bacteria on eosinophil activation measured by a colorimetric assay with o-phenylenediamine (OPD) substrate. We observed a higher number of eosinophils and increased extracellular ECP in the mucosa of IBS patients compared with HC. Moreover, F-EDN levels in IBS samples were elevated compared with HC and positively correlated to extracellular ECP. Metagenomic analysis showed significant correlations between bacterial composition and eosinophil measurements in both HC and IBS patients. In vitro experiments revealed an increased degranulation of 15HL-60 after stimulation with Salmonella typhimurium, Salmonella enterica, and Yersinia enterocolitica. To conclude, we could demonstrate alterations related to eosinophils in IBS, and, for the first time, a positive correlation between F-EDN levels and degranulated eosinophils in the colonic mucosa of IBS patients. Together our results suggest that eosinophils play a role in the pathophysiology of IBS and the mechanisms might be linked to an altered microbiota.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Bacteria
- eosinophil cationic protein
- fecal eosinophil-derived neurotoxin
- irritable bowel syndrome
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Casado-Bedmar, M ...
-
Meira de Faria, ...
-
Biskou, Olga
-
Lindqvist, Carl ...
-
Ranasinghe, Purn ...
-
Bednarska, Olga
-
show more...
-
Peterson, Christ ...
-
Walter, Susanna
-
Carlsson, Maria ...
-
Keita, Åsa
-
Casado Bedmar, M ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Clinical Laborat ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Gastroenterology ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
- Articles in the publication
-
Journal of Leuko ...
- By the university
-
Örebro University
-
Uppsala University
-
Linköping University