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Combined Magnetolip...
Combined Magnetoliposome Formation and Drug Loading in One Step for Efficient Alternating Current-Magnetic Field Remote-Controlled Drug Release.
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- Fortes Brollo, Maria Eugenia (author)
- Instituto de Ciencia de Materiales de Madrid, Spain
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- Domínguez-Bajo, Ana (author)
- Instituto de Ciencia de Materiales de Madrid, Spain
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- Tabero, Andrea (author)
- Universidad Autónoma de Madrid, Spain
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- Domínguez-Arca, Vicente (author)
- Universidad de Santiago de Compostela, Spain
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- Gisbert, Victor (author)
- Instituto de Ciencia de Materiales de Madrid, Spain
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- Prieto, Gerardo (author)
- Universidad de Santiago de Compostela, Spain
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- Johansson, Christer (author)
- RISE,Smart hårdvara
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- Garcia, Ricardo (author)
- Instituto de Ciencia de Materiales de Madrid, Spain
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- Villanueva, Angeles (author)
- Universidad Autónoma de Madrid, Spain
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- Serrano, María Concepción (author)
- Instituto de Ciencia de Materiales de Madrid, Spain
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- Morales, María Del Puerto (author)
- Instituto de Ciencia de Materiales de Madrid, Spain; IMDEA-Nanociencia, Spain
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(creator_code:org_t)
- 2020-01-06
- 2020
- English.
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In: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 12:4, s. 4295-4307
- Related links:
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https://repositorio....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- We have developed a reproducible and facile one step strategy for the synthesis of doxorubicin loaded magnetoliposomes by using a thin-layer evaporation method. Liposomes of around 200 nm were made of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and iron oxide nanoparticles (NPs) with negative, positive, and hydrophobic surfaces that were incorporated outside, inside, or between the lipid bilayers, respectively. To characterize how NPs are incorporated in liposomes, advanced cryoTEM and atomic force microscope (AFM) techniques have been used. It was observed that only when the NPs are attached outside the liposomes, the membrane integrity is preserved (lipid melt transition shifts to 38.7 °C with high enthalpy 34.8 J/g) avoiding the leakage of the encapsulated drug while having good colloidal properties and the best heating efficiency under an alternating magnetic field (AMF). These magnetoliposomes were tested with two cancer cell lines, MDA-MB-231 and HeLa cells. First, 100% of cellular uptake was achieved with a high cell survival (above 80%), which is preserved (83%) for doxorubicin-loaded magnetoliposomes. Then, we demonstrate that doxorubicin release can be triggered by remote control, using a noninvasive external AMF for 1 h, leading to a cell survival reduction of 20%. Magnetic field conditions of 202 kHz and 30 mT seem to be enough to produce an effective heating to avoid drug degradation. In conclusion, these drug-loaded magnetoliposomes prepared in one step could be used for drug release on demand at a specific time and place, efficiently using an external AMF to reduce or even eliminate side effects.
Keyword
- HeLa cells
- MDA-MB-231 cells
- doxorubicin
- drug delivery carrier
- magnetic hyperthermia
- magnetoliposomes
- superparamagnetic iron oxide nanoparticle
Publication and Content Type
- ref (subject category)
- art (subject category)
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Fortes Brollo, M ...
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Domínguez-Bajo, ...
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Tabero, Andrea
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Domínguez-Arca, ...
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Gisbert, Victor
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Prieto, Gerardo
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Johansson, Chris ...
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Garcia, Ricardo
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Villanueva, Ange ...
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Serrano, María C ...
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Morales, María D ...
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ACS Applied Mate ...
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